Sequential injection Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme

A sequential injection-Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme was developed. Air segments were introduced for separation of enzyme and substrate zones and separation of the stacked zones from the carrier solution which ensure the measurement of the initial rate an...

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Main Authors: Kraingkrai Ponhong, Supaporn Kradtap Hartwell, Kate Grudpan
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/49818
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-498182018-09-04T04:18:38Z Sequential injection Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme Kraingkrai Ponhong Supaporn Kradtap Hartwell Kate Grudpan Chemistry A sequential injection-Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme was developed. Air segments were introduced for separation of enzyme and substrate zones and separation of the stacked zones from the carrier solution which ensure the measurement of the initial rate and minimize the dilution/dispersion effect. The open- ended mixing chamber makes it possible to use air segments in the flow system without the need for additional air segment discarding steps. The enzyme horseradish peroxidase (HRP) kinetic parameters based on initial rate was used as a model study. The operation of the system is virtually the same as that of the conventional batch-wise process. The kinetic parameters (i.e. Kmand Vmax) of HRP obtained using the proposed system agree well with those obtained using the batch-wise process as well. The proposed system offers additional benefits of volume down scaling, improved rapidity and automatic features that does not require a skillful operator. © 2011 Elsevier B.V. All rights reserved. 2018-09-04T04:18:38Z 2018-09-04T04:18:38Z 2011-07-15 Journal 00399140 2-s2.0-79958102298 10.1016/j.talanta.2011.03.086 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79958102298&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49818
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
spellingShingle Chemistry
Kraingkrai Ponhong
Supaporn Kradtap Hartwell
Kate Grudpan
Sequential injection Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme
description A sequential injection-Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme was developed. Air segments were introduced for separation of enzyme and substrate zones and separation of the stacked zones from the carrier solution which ensure the measurement of the initial rate and minimize the dilution/dispersion effect. The open- ended mixing chamber makes it possible to use air segments in the flow system without the need for additional air segment discarding steps. The enzyme horseradish peroxidase (HRP) kinetic parameters based on initial rate was used as a model study. The operation of the system is virtually the same as that of the conventional batch-wise process. The kinetic parameters (i.e. Kmand Vmax) of HRP obtained using the proposed system agree well with those obtained using the batch-wise process as well. The proposed system offers additional benefits of volume down scaling, improved rapidity and automatic features that does not require a skillful operator. © 2011 Elsevier B.V. All rights reserved.
format Journal
author Kraingkrai Ponhong
Supaporn Kradtap Hartwell
Kate Grudpan
author_facet Kraingkrai Ponhong
Supaporn Kradtap Hartwell
Kate Grudpan
author_sort Kraingkrai Ponhong
title Sequential injection Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme
title_short Sequential injection Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme
title_full Sequential injection Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme
title_fullStr Sequential injection Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme
title_full_unstemmed Sequential injection Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme
title_sort sequential injection lab-at-valve (si-lav) segmented flow system for kinetic study of an enzyme
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79958102298&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/49818
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