Theoretical analysis on ratiometric fluorescent indicators caused biased estimates of intracellular free calcium concentrations

• Main Authors: Hu Z., Yu L., Yu Z.
• Format: Review
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-13544260798&partnerID=40&md5=7bc7fb514f1b4a5919f85407a4f0a2a3; http://cmuir.cmu.ac.th/handle/6653943832/5001
• Institution: Chiang Mai University

Ratiometric fluorescent calcium indicator dyes have been widely used for the study of the role of Ca2+ in cell physiopathology. Although these ratiometric dyes offer several advantages over others, they suffer some drawbacks which cause serious errors in measurement of intracellular Ca2+ concentration, [Ca2+i. The present study systematically analyzes theoretical reasons and technical sources of discrepancies occurring in the measurement of the characteristics of the agonists-induced cells [Ca2+i. In order to avoid the errors and achieve the accurate determination of [Ca2+]i, this study proposes solutions and suggests some critical measures in both theoretical and technical aspects. Therefore, this analysis can be a valuable tool in clarifying proper usages of fluorescent dyes for [Ca2+i measurements. © 2004 Elsevier B.V. All rights reserved.