A single-tube multiplex PCR for rapid detection in feces of 10 viruses causing diarrhea

• Main Authors: Pattara Khamrin, Makiko Okame, Aksara Thongprachum, Nattika Nantachit, Shuichi Nishimura, Shoko Okitsu, Niwat Maneekarn, Hiroshi Ushijima
• Format: Journal
• Published: 2018
• Subjects: Immunology and Microbiology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79954573776&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/50031
• Institution: Chiang Mai University

A novel multiplex polymerase chain reaction assay was developed to identify 10 viruses in a single tube. The assay was targeted to detect group A and C rotaviruses, adenovirus, norovirus GI, norovirus GII, sapovirus, astrovirus, Aichi virus, parechovirus, and enterovirus. A total of 235 stool sample...