Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos

Objective: To study the effect of embryo density and microdrop volume on mouse two-cell embryo development. Design: Experimental study. Setting: Assisted conception laboratory. Animal(s): Two-cell mouse embryos (n = 1511). Intervention(s): One, five, 10, or 15 embryos were cultured in 10-μL drops of...

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Main Authors: Teraporn Vutyavanich, Ubol Saeng-Anan, Suppachai Sirisukkasem, Waraporn Piromlertamorn
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/50255
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-502552018-09-04T04:27:18Z Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos Teraporn Vutyavanich Ubol Saeng-Anan Suppachai Sirisukkasem Waraporn Piromlertamorn Medicine Objective: To study the effect of embryo density and microdrop volume on mouse two-cell embryo development. Design: Experimental study. Setting: Assisted conception laboratory. Animal(s): Two-cell mouse embryos (n = 1511). Intervention(s): One, five, 10, or 15 embryos were cultured in 10-μL drops of cleavage medium. In the second study, embryos were cultured singly in 0.5-, 1-, 2-, 5-, and 10-μL drops. Finally, they were cultured in pair in 0.5-, 1-, and 2-μL drops. After 48 hours, embryos were transferred into blastocyst medium for an additional 24 hours. Main Outcome Measure(s): Cleavage and blastocyst formation and inner cell mass (ICM) and trophectoderm (TE) cell numbers. Result(s): No differences in cleavage or blastocyst formation were found in different groups in experiment 1, 2, or 3. Embryos cultured singly had fewer ICM and TE cells than those cultured in groups. Embryos cultured singly in 0.5 μL had fewer TE cells than those in 10 μL, but had insignificant difference in the ICM. Duo culture in 0.5-2 μL appeared to give the same results as group culture in 10-μL drops. Conclusion(s): Group culture is preferred when using sequential media. Beneficial effects cannot be mimicked by volume reduction in single-embryo culture. © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc. 2018-09-04T04:27:18Z 2018-09-04T04:27:18Z 2011-03-15 Journal 00150282 2-s2.0-79952448537 10.1016/j.fertnstert.2010.05.005 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79952448537&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50255
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Teraporn Vutyavanich
Ubol Saeng-Anan
Suppachai Sirisukkasem
Waraporn Piromlertamorn
Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
description Objective: To study the effect of embryo density and microdrop volume on mouse two-cell embryo development. Design: Experimental study. Setting: Assisted conception laboratory. Animal(s): Two-cell mouse embryos (n = 1511). Intervention(s): One, five, 10, or 15 embryos were cultured in 10-μL drops of cleavage medium. In the second study, embryos were cultured singly in 0.5-, 1-, 2-, 5-, and 10-μL drops. Finally, they were cultured in pair in 0.5-, 1-, and 2-μL drops. After 48 hours, embryos were transferred into blastocyst medium for an additional 24 hours. Main Outcome Measure(s): Cleavage and blastocyst formation and inner cell mass (ICM) and trophectoderm (TE) cell numbers. Result(s): No differences in cleavage or blastocyst formation were found in different groups in experiment 1, 2, or 3. Embryos cultured singly had fewer ICM and TE cells than those cultured in groups. Embryos cultured singly in 0.5 μL had fewer TE cells than those in 10 μL, but had insignificant difference in the ICM. Duo culture in 0.5-2 μL appeared to give the same results as group culture in 10-μL drops. Conclusion(s): Group culture is preferred when using sequential media. Beneficial effects cannot be mimicked by volume reduction in single-embryo culture. © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc.
format Journal
author Teraporn Vutyavanich
Ubol Saeng-Anan
Suppachai Sirisukkasem
Waraporn Piromlertamorn
author_facet Teraporn Vutyavanich
Ubol Saeng-Anan
Suppachai Sirisukkasem
Waraporn Piromlertamorn
author_sort Teraporn Vutyavanich
title Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
title_short Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
title_full Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
title_fullStr Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
title_full_unstemmed Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
title_sort effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79952448537&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50255
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