Development of HAT-RAPD marker for detection of stellantchasmus falcatus infection

The trematode, Stellantchasmus falcatus, is one of the members in the Family Heterophyidae, which is reported to be an endemic fluke in northern region of Thailand. A sensitive and specific detection of this parasite is required to determine the epidemiology at larval stages. Specific primers to det...

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Main Author: Chalobol Wongsawad
Format: Journal
Published: 2018
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Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79953762751&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50287
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-502872018-09-04T04:27:48Z Development of HAT-RAPD marker for detection of stellantchasmus falcatus infection Chalobol Wongsawad Medicine The trematode, Stellantchasmus falcatus, is one of the members in the Family Heterophyidae, which is reported to be an endemic fluke in northern region of Thailand. A sensitive and specific detection of this parasite is required to determine the epidemiology at larval stages. Specific primers to determine the presence of S. falcatus were investigated using high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR, with 10 arbitrary primers to generate different polymorphic DNA profiles. Eleven parasite species were used for comparison. A 380 bp HAT-RAPD S. falcatus-specific marker was found, and was cloned and sequenced, allowing a pair of primers (St-F 5'-GGCCAACG CAATCGTCATCC-3' and St-R 5'-GCGTCGGGTTTCAGACATGG- 3') to be designed to produce a 320 bp amplicon specific for S. falcatus. It revealed no crossreaction with any of the other tested parasite species. The S. falcatus-specific primers can be used for epidemiological monitoring and for detection in snail intermediate hosts, which serve as usefulness tools in management and epidemiological control programs. 2018-09-04T04:27:48Z 2018-09-04T04:27:48Z 2011-01-01 Journal 01251562 2-s2.0-79953762751 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79953762751&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50287
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Chalobol Wongsawad
Development of HAT-RAPD marker for detection of stellantchasmus falcatus infection
description The trematode, Stellantchasmus falcatus, is one of the members in the Family Heterophyidae, which is reported to be an endemic fluke in northern region of Thailand. A sensitive and specific detection of this parasite is required to determine the epidemiology at larval stages. Specific primers to determine the presence of S. falcatus were investigated using high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR, with 10 arbitrary primers to generate different polymorphic DNA profiles. Eleven parasite species were used for comparison. A 380 bp HAT-RAPD S. falcatus-specific marker was found, and was cloned and sequenced, allowing a pair of primers (St-F 5'-GGCCAACG CAATCGTCATCC-3' and St-R 5'-GCGTCGGGTTTCAGACATGG- 3') to be designed to produce a 320 bp amplicon specific for S. falcatus. It revealed no crossreaction with any of the other tested parasite species. The S. falcatus-specific primers can be used for epidemiological monitoring and for detection in snail intermediate hosts, which serve as usefulness tools in management and epidemiological control programs.
format Journal
author Chalobol Wongsawad
author_facet Chalobol Wongsawad
author_sort Chalobol Wongsawad
title Development of HAT-RAPD marker for detection of stellantchasmus falcatus infection
title_short Development of HAT-RAPD marker for detection of stellantchasmus falcatus infection
title_full Development of HAT-RAPD marker for detection of stellantchasmus falcatus infection
title_fullStr Development of HAT-RAPD marker for detection of stellantchasmus falcatus infection
title_full_unstemmed Development of HAT-RAPD marker for detection of stellantchasmus falcatus infection
title_sort development of hat-rapd marker for detection of stellantchasmus falcatus infection
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79953762751&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50287
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