Potent enhancement of GFP uptake into HT-29 cells and rat skin permeation by coincubation with tat peptide

The delivery enhancements of green fluorescent protein (GFP), a model reporter protein into/transepithelial colon adenocarcinoma (HT-29) cells and excised rat skin by coincubation, by simple mixing or as fusion protein with HIV1-trans-activating transcriptional (Tat), a cell-penetrating peptide, hav...

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Main Authors: Warangkana Lohcharoenkal, Aranya Manosaroi, Friedrich Götz, Rolf G. Werner, Worapaka Manosroi, Jiradej Manosaroi
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/50333
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-503332018-09-04T04:29:12Z Potent enhancement of GFP uptake into HT-29 cells and rat skin permeation by coincubation with tat peptide Warangkana Lohcharoenkal Aranya Manosaroi Friedrich Götz Rolf G. Werner Worapaka Manosroi Jiradej Manosaroi Pharmacology, Toxicology and Pharmaceutics The delivery enhancements of green fluorescent protein (GFP), a model reporter protein into/transepithelial colon adenocarcinoma (HT-29) cells and excised rat skin by coincubation, by simple mixing or as fusion protein with HIV1-trans-activating transcriptional (Tat), a cell-penetrating peptide, have been described. By simple mixing, Tat/GFP mixture could increase the cellular uptake of GFP into HT-29 cells by 4.25-fold and 1.79-fold of GFP and Tat-GFP fusion protein, respectively. The incubation time showed no effect on the cellular uptake of Tat/GFP and Tat-GFP. In transepithelial study, Tat-GFP demonstrated the highest ability to penetrate through the HT-29 cells of about 1.3-fold and 1.2-fold of GFP and Tat/GFP, respectively. Only Tat/GFP gave lower cytotoxicity than Tat or GFP alone. In transdermal delivery study, Tat/GFP showed better transdermal delivery profile with higher cumulative amount than Tat-GFP in stratum corneum (SC), viable epidermis and dermis, and the receiver compartment of the diffusion cell with the highest fluxes of 7.42 and 35.6; 8.87-fold and 5.57-fold of GFP and Tat-GFP in SC and receiver compartment, respectively. This study demonstrated an efficient enhancement of GFP uptake into cells and through excised rat skin by simple mixing with Tat peptide, which can be further applied for the development of protein drug delivery systems. © 2011 Wiley-Liss, Inc. 2018-09-04T04:29:12Z 2018-09-04T04:29:12Z 2011-01-01 Journal 15206017 00223549 2-s2.0-80053287585 10.1002/jps.22671 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80053287585&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50333
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Pharmacology, Toxicology and Pharmaceutics
spellingShingle Pharmacology, Toxicology and Pharmaceutics
Warangkana Lohcharoenkal
Aranya Manosaroi
Friedrich Götz
Rolf G. Werner
Worapaka Manosroi
Jiradej Manosaroi
Potent enhancement of GFP uptake into HT-29 cells and rat skin permeation by coincubation with tat peptide
description The delivery enhancements of green fluorescent protein (GFP), a model reporter protein into/transepithelial colon adenocarcinoma (HT-29) cells and excised rat skin by coincubation, by simple mixing or as fusion protein with HIV1-trans-activating transcriptional (Tat), a cell-penetrating peptide, have been described. By simple mixing, Tat/GFP mixture could increase the cellular uptake of GFP into HT-29 cells by 4.25-fold and 1.79-fold of GFP and Tat-GFP fusion protein, respectively. The incubation time showed no effect on the cellular uptake of Tat/GFP and Tat-GFP. In transepithelial study, Tat-GFP demonstrated the highest ability to penetrate through the HT-29 cells of about 1.3-fold and 1.2-fold of GFP and Tat/GFP, respectively. Only Tat/GFP gave lower cytotoxicity than Tat or GFP alone. In transdermal delivery study, Tat/GFP showed better transdermal delivery profile with higher cumulative amount than Tat-GFP in stratum corneum (SC), viable epidermis and dermis, and the receiver compartment of the diffusion cell with the highest fluxes of 7.42 and 35.6; 8.87-fold and 5.57-fold of GFP and Tat-GFP in SC and receiver compartment, respectively. This study demonstrated an efficient enhancement of GFP uptake into cells and through excised rat skin by simple mixing with Tat peptide, which can be further applied for the development of protein drug delivery systems. © 2011 Wiley-Liss, Inc.
format Journal
author Warangkana Lohcharoenkal
Aranya Manosaroi
Friedrich Götz
Rolf G. Werner
Worapaka Manosroi
Jiradej Manosaroi
author_facet Warangkana Lohcharoenkal
Aranya Manosaroi
Friedrich Götz
Rolf G. Werner
Worapaka Manosroi
Jiradej Manosaroi
author_sort Warangkana Lohcharoenkal
title Potent enhancement of GFP uptake into HT-29 cells and rat skin permeation by coincubation with tat peptide
title_short Potent enhancement of GFP uptake into HT-29 cells and rat skin permeation by coincubation with tat peptide
title_full Potent enhancement of GFP uptake into HT-29 cells and rat skin permeation by coincubation with tat peptide
title_fullStr Potent enhancement of GFP uptake into HT-29 cells and rat skin permeation by coincubation with tat peptide
title_full_unstemmed Potent enhancement of GFP uptake into HT-29 cells and rat skin permeation by coincubation with tat peptide
title_sort potent enhancement of gfp uptake into ht-29 cells and rat skin permeation by coincubation with tat peptide
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80053287585&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50333
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