Pulsed amperometry for anti-fouling of boron-doped diamond in electroanalysis of β-agonists: Application to flow injection for pharmaceutical analysis

• Main Authors: Karuwan C., Mantim T., Chaisuwan P., Wilairat P., Grudpan K., Jittangprasert P., Einaga Y., Chailapakul O., Suntornsuk L., Anurukvorakun O., Nacapricha D.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-33947367459&partnerID=40&md5=f4d68821651d030e9e1cd637ada2143f; http://cmuir.cmu.ac.th/handle/6653943832/5034
• Institution: Chiang Mai University

This work presents the construction and application of boron-doped diamond (BDD) thin film electrode as sensor for the determination of three β-agonists, viz. salbutamol, terbutaline and clenbuterol. Although well-known as a chemically inert material, BDD film however shows fouling in detection of these compounds using fixed-potential mode amperometry. A suitable waveform for pulsed amperometric detection (PAD) was developed and used to determine the agonist compounds. It was seen that the developed PAD significantly refreshed the BDD surface for long-term detection in flow injection analysis. Linear working ranges were 0.5-100 μM, 1.0-100 μM and 0.5-50 μM for salbutamol, terbutaline and clenbuterol, respectively. The developed PAD-BDD system was applied to successfully determine salbutamol and terbutaline in commercial pharmaceutical products. The methods were validated with a capillary electrophoresis method. © 2006 by MDPI.