Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059

Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059

A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and...

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Main Authors: Nattawooti Sthitmatee, Yasushi Kataoka, Takuo Sawada
Format: Journal
Published: 2018
Subjects:
Veterinary
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=82955251111&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50371
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-503712018-09-04T04:31:20Z Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059 Nattawooti Sthitmatee Yasushi Kataoka Takuo Sawada Veterinary A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroscope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens. 2018-09-04T04:31:20Z 2018-09-04T04:31:20Z 2011-11-01 Journal 13477439 09167250 2-s2.0-82955251111 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=82955251111&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50371
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Veterinary
spellingShingle Veterinary
Nattawooti Sthitmatee
Yasushi Kataoka
Takuo Sawada
Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059
description A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroscope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens.
format Journal
author Nattawooti Sthitmatee
Yasushi Kataoka
Takuo Sawada
author_facet Nattawooti Sthitmatee
Yasushi Kataoka
Takuo Sawada
author_sort Nattawooti Sthitmatee
title Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059
title_short Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059
title_full Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059
title_fullStr Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059
title_full_unstemmed Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059
title_sort inhibition of capsular protein synthesis of pasteurella multocida strain p-1059
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=82955251111&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50371
_version_ 1681423577805488128

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