A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance

Sodium channel mutations were investigated through nucleotide sequencing of three cDNA fragments amplified from permethrin resistant and susceptible Aedes aegypti from northern Thailand. There was a novel nucleotide substitution (T → G) at the second position of codon 1552 resulting in the replaceme...

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Main Authors: Jintana Yanola, Pradya Somboon, Catherine Walton, Woottichai Nachaiwieng, La aied Prapanthadara
Format: Journal
Published: 2018
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spelling th-cmuir.6653943832-504872018-09-04T04:47:10Z A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance Jintana Yanola Pradya Somboon Catherine Walton Woottichai Nachaiwieng La aied Prapanthadara Agricultural and Biological Sciences Environmental Science Sodium channel mutations were investigated through nucleotide sequencing of three cDNA fragments amplified from permethrin resistant and susceptible Aedes aegypti from northern Thailand. There was a novel nucleotide substitution (T → G) at the second position of codon 1552 resulting in the replacement of Phenylalanine by Cysteine in segment 6 domain III. This amino acid was indicated by another study to involve an aromatic-aromatic contact between the sodium channel protein and the first aromatic ring of the pyrethroid alcohol moiety. Reciprocal crosses between the homozygous parental susceptible and resistant strains indicated that resistance was autosomal and incompletely recessive, and highly associated with the homozygous mutation. The bioassay of the F2progeny, formed by backcrossing the F1with the resistant parental strain, did not show a clear plateau curve across the range of doses, suggesting that resistance to permethrin was controlled by more than one gene locus. Other possible resistance mechanisms involved are discussed. © 2009 Elsevier Inc. All rights reserved. 2018-09-04T04:41:30Z 2018-09-04T04:41:30Z 2010-03-01 Journal 10959939 00483575 2-s2.0-77349102469 10.1016/j.pestbp.2009.10.005 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77349102469&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50487
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Environmental Science
spellingShingle Agricultural and Biological Sciences
Environmental Science
Jintana Yanola
Pradya Somboon
Catherine Walton
Woottichai Nachaiwieng
La aied Prapanthadara
A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance
description Sodium channel mutations were investigated through nucleotide sequencing of three cDNA fragments amplified from permethrin resistant and susceptible Aedes aegypti from northern Thailand. There was a novel nucleotide substitution (T → G) at the second position of codon 1552 resulting in the replacement of Phenylalanine by Cysteine in segment 6 domain III. This amino acid was indicated by another study to involve an aromatic-aromatic contact between the sodium channel protein and the first aromatic ring of the pyrethroid alcohol moiety. Reciprocal crosses between the homozygous parental susceptible and resistant strains indicated that resistance was autosomal and incompletely recessive, and highly associated with the homozygous mutation. The bioassay of the F2progeny, formed by backcrossing the F1with the resistant parental strain, did not show a clear plateau curve across the range of doses, suggesting that resistance to permethrin was controlled by more than one gene locus. Other possible resistance mechanisms involved are discussed. © 2009 Elsevier Inc. All rights reserved.
format Journal
author Jintana Yanola
Pradya Somboon
Catherine Walton
Woottichai Nachaiwieng
La aied Prapanthadara
author_facet Jintana Yanola
Pradya Somboon
Catherine Walton
Woottichai Nachaiwieng
La aied Prapanthadara
author_sort Jintana Yanola
title A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance
title_short A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance
title_full A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance
title_fullStr A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance
title_full_unstemmed A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance
title_sort novel f1552/c1552 point mutation in the aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77349102469&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50487
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