Molecular modeling of peroxidase and polyphenol Oxidase: Substrate specificity and active site comparison
Peroxidases (POD) and polyphenol oxidase (PPO) are enzymes that are well known to be involved in the enzymatic browning reaction of fruits and vegetables with different catalytic mechanisms. Both enzymes have some common substrates, but each also has its specific substrates. In our computational stu...
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th-cmuir.6653943832-505432018-09-04T04:44:37Z Molecular modeling of peroxidase and polyphenol Oxidase: Substrate specificity and active site comparison Prontipa Nokthai Vannajan Sanghiran Lee Lalida Shank Biochemistry, Genetics and Molecular Biology Chemical Engineering Chemistry Computer Science Peroxidases (POD) and polyphenol oxidase (PPO) are enzymes that are well known to be involved in the enzymatic browning reaction of fruits and vegetables with different catalytic mechanisms. Both enzymes have some common substrates, but each also has its specific substrates. In our computational study, the amino acid sequence of grape peroxidase (ABX) was used for the construction of models employing homology modeling method based on the X-ray structure of cytosolic ascorbate peroxidase from pea (PDB ID:1APX), whereas the model of grape polyphenol oxidase was obtained directly from the available X-ray structure (PDB ID:2P3X). Molecular docking of common substrates of these two enzymes was subsequently studied. It was found that epicatechin and catechin exhibited high affinity with both enzymes, even though POD and PPO have different binding pockets regarding the size and the key amino acids involved in binding. Predicted binding modes of substrates with both enzymes were also compared. The calculated docking interaction energy of trihydroxybenzoic acid related compounds shows high affinity, suggesting specificity and potential use as common inhibitor to grape ascorbate peroxidase and polyphenol oxidase. © 2010 by the authors; licensee MDPI, Basel, Switzerland. 2018-09-04T04:42:08Z 2018-09-04T04:42:08Z 2010-09-01 Journal 14220067 2-s2.0-77958530134 10.3390/ijms11093266 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77958530134&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50543 |
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Biochemistry, Genetics and Molecular Biology Chemical Engineering Chemistry Computer Science Prontipa Nokthai Vannajan Sanghiran Lee Lalida Shank Molecular modeling of peroxidase and polyphenol Oxidase: Substrate specificity and active site comparison |
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Peroxidases (POD) and polyphenol oxidase (PPO) are enzymes that are well known to be involved in the enzymatic browning reaction of fruits and vegetables with different catalytic mechanisms. Both enzymes have some common substrates, but each also has its specific substrates. In our computational study, the amino acid sequence of grape peroxidase (ABX) was used for the construction of models employing homology modeling method based on the X-ray structure of cytosolic ascorbate peroxidase from pea (PDB ID:1APX), whereas the model of grape polyphenol oxidase was obtained directly from the available X-ray structure (PDB ID:2P3X). Molecular docking of common substrates of these two enzymes was subsequently studied. It was found that epicatechin and catechin exhibited high affinity with both enzymes, even though POD and PPO have different binding pockets regarding the size and the key amino acids involved in binding. Predicted binding modes of substrates with both enzymes were also compared. The calculated docking interaction energy of trihydroxybenzoic acid related compounds shows high affinity, suggesting specificity and potential use as common inhibitor to grape ascorbate peroxidase and polyphenol oxidase. © 2010 by the authors; licensee MDPI, Basel, Switzerland. |
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Prontipa Nokthai Vannajan Sanghiran Lee Lalida Shank |
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Prontipa Nokthai Vannajan Sanghiran Lee Lalida Shank |
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Prontipa Nokthai |
title |
Molecular modeling of peroxidase and polyphenol Oxidase: Substrate specificity and active site comparison |
title_short |
Molecular modeling of peroxidase and polyphenol Oxidase: Substrate specificity and active site comparison |
title_full |
Molecular modeling of peroxidase and polyphenol Oxidase: Substrate specificity and active site comparison |
title_fullStr |
Molecular modeling of peroxidase and polyphenol Oxidase: Substrate specificity and active site comparison |
title_full_unstemmed |
Molecular modeling of peroxidase and polyphenol Oxidase: Substrate specificity and active site comparison |
title_sort |
molecular modeling of peroxidase and polyphenol oxidase: substrate specificity and active site comparison |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77958530134&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50543 |
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