Sequential injection-cation exchange micro-column system for hemoglobin typing to differentiate HbE carriers

A weak cation exchange micro-column was incorporated into a sequential injection (SI) system to perform automatic hemoglobin (Hb) typing as an alternative way to measure HbE. Separation of HbF, HbA and HbA2/HbE was performed using phosphate buffer solutions in the pH range of 6 - 7 to create pH grad...

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Main Authors: Supaporn Kradtap Hartwell, Worathip Sripaoraya, Somchai Lapanantnoppakhun, Torpong Sanguansermsri, Kate Grudpan
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/50680
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-506802018-09-04T04:51:36Z Sequential injection-cation exchange micro-column system for hemoglobin typing to differentiate HbE carriers Supaporn Kradtap Hartwell Worathip Sripaoraya Somchai Lapanantnoppakhun Torpong Sanguansermsri Kate Grudpan Chemistry Medicine A weak cation exchange micro-column was incorporated into a sequential injection (SI) system to perform automatic hemoglobin (Hb) typing as an alternative way to measure HbE. Separation of HbF, HbA and HbA2/HbE was performed using phosphate buffer solutions in the pH range of 6 - 7 to create pH gradient mobile phase. The resultant chromatogram showed relative amounts of HbE to other types of hemoglobins in more quantitative detail than the conventional techniques such as dichlorophenol indophenol precipitation and micro-column anion exchange. The system is more economical than a commercially available ion-exchange HPLC analyzer for hemoglobin testing, though analysis time per run is longer due to the aspiration operation of the syringe pump of the SI system. It has been demonstrated that the system can differentiate negative (normal) from positive (HbE carriers) subjects. 2010 © The Japan Society for Analytical Chemistry. 2018-09-04T04:44:04Z 2018-09-04T04:44:04Z 2010-03-01 Journal 13482246 09106340 2-s2.0-77953633916 10.2116/analsci.26.361 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77953633916&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50680
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
Medicine
spellingShingle Chemistry
Medicine
Supaporn Kradtap Hartwell
Worathip Sripaoraya
Somchai Lapanantnoppakhun
Torpong Sanguansermsri
Kate Grudpan
Sequential injection-cation exchange micro-column system for hemoglobin typing to differentiate HbE carriers
description A weak cation exchange micro-column was incorporated into a sequential injection (SI) system to perform automatic hemoglobin (Hb) typing as an alternative way to measure HbE. Separation of HbF, HbA and HbA2/HbE was performed using phosphate buffer solutions in the pH range of 6 - 7 to create pH gradient mobile phase. The resultant chromatogram showed relative amounts of HbE to other types of hemoglobins in more quantitative detail than the conventional techniques such as dichlorophenol indophenol precipitation and micro-column anion exchange. The system is more economical than a commercially available ion-exchange HPLC analyzer for hemoglobin testing, though analysis time per run is longer due to the aspiration operation of the syringe pump of the SI system. It has been demonstrated that the system can differentiate negative (normal) from positive (HbE carriers) subjects. 2010 © The Japan Society for Analytical Chemistry.
format Journal
author Supaporn Kradtap Hartwell
Worathip Sripaoraya
Somchai Lapanantnoppakhun
Torpong Sanguansermsri
Kate Grudpan
author_facet Supaporn Kradtap Hartwell
Worathip Sripaoraya
Somchai Lapanantnoppakhun
Torpong Sanguansermsri
Kate Grudpan
author_sort Supaporn Kradtap Hartwell
title Sequential injection-cation exchange micro-column system for hemoglobin typing to differentiate HbE carriers
title_short Sequential injection-cation exchange micro-column system for hemoglobin typing to differentiate HbE carriers
title_full Sequential injection-cation exchange micro-column system for hemoglobin typing to differentiate HbE carriers
title_fullStr Sequential injection-cation exchange micro-column system for hemoglobin typing to differentiate HbE carriers
title_full_unstemmed Sequential injection-cation exchange micro-column system for hemoglobin typing to differentiate HbE carriers
title_sort sequential injection-cation exchange micro-column system for hemoglobin typing to differentiate hbe carriers
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77953633916&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50680
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