Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dep...
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2014
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th-cmuir.6653943832-50822014-08-30T02:56:08Z Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation Nitsawang S. Hatti-Kaul R. Kanasawud P. Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. © 2006 Elsevier Inc. All rights reserved. 2014-08-30T02:56:08Z 2014-08-30T02:56:08Z 2006 Article 01410229 10.1016/j.enzmictec.2006.02.013 EMTED http://www.scopus.com/inward/record.url?eid=2-s2.0-33745959045&partnerID=40&md5=9aba8a6df523af8726edeac5a44d3daf http://cmuir.cmu.ac.th/handle/6653943832/5082 English |
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Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. © 2006 Elsevier Inc. All rights reserved. |
| format |
Article |
| author |
Nitsawang S. Hatti-Kaul R. Kanasawud P. |
| spellingShingle |
Nitsawang S. Hatti-Kaul R. Kanasawud P. Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation |
| author_facet |
Nitsawang S. Hatti-Kaul R. Kanasawud P. |
| author_sort |
Nitsawang S. |
| title |
Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation |
| title_short |
Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation |
| title_full |
Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation |
| title_fullStr |
Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation |
| title_full_unstemmed |
Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation |
| title_sort |
purification of papain from carica papaya latex: aqueous two-phase extraction versus two-step salt precipitation |
| publishDate |
2014 |
| url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-33745959045&partnerID=40&md5=9aba8a6df523af8726edeac5a44d3daf http://cmuir.cmu.ac.th/handle/6653943832/5082 |
| _version_ |
1681420358222086144 |