$Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation$

Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation

Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dep...

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Main Authors:	Nitsawang S., Hatti-Kaul R., Kanasawud P.
Format:	Article
Language:	English
Published:	2014
Online Access:	http://www.scopus.com/inward/record.url?eid=2-s2.0-33745959045&partnerID=40&md5=9aba8a6df523af8726edeac5a44d3daf http://cmuir.cmu.ac.th/handle/6653943832/5082
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Institution:	Chiang Mai University
Language:	English

id	th-cmuir.6653943832-5082
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spelling	th-cmuir.6653943832-50822014-08-30T02:56:08Z Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation Nitsawang S. Hatti-Kaul R. Kanasawud P. Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. © 2006 Elsevier Inc. All rights reserved. 2014-08-30T02:56:08Z 2014-08-30T02:56:08Z 2006 Article 01410229 10.1016/j.enzmictec.2006.02.013 EMTED http://www.scopus.com/inward/record.url?eid=2-s2.0-33745959045&partnerID=40&md5=9aba8a6df523af8726edeac5a44d3daf http://cmuir.cmu.ac.th/handle/6653943832/5082 English
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
language	English
description	Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. © 2006 Elsevier Inc. All rights reserved.
format	Article
author	Nitsawang S. Hatti-Kaul R. Kanasawud P.
spellingShingle	Nitsawang S. Hatti-Kaul R. Kanasawud P. Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
author_facet	Nitsawang S. Hatti-Kaul R. Kanasawud P.
author_sort	Nitsawang S.
title	Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
title_short	Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
title_full	Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
title_fullStr	Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
title_full_unstemmed	Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
title_sort	purification of papain from carica papaya latex: aqueous two-phase extraction versus two-step salt precipitation
publishDate	2014
url	http://www.scopus.com/inward/record.url?eid=2-s2.0-33745959045&partnerID=40&md5=9aba8a6df523af8726edeac5a44d3daf http://cmuir.cmu.ac.th/handle/6653943832/5082
_version_	1681420358222086144

Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation

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