Gnathostoma spinigerum: Molecular cloning, expression and characterization of the cyclophilin protein

Gnathostoma spinigerum: Molecular cloning, expression and characterization of the cyclophilin protein

In this study, a cDNA encoding cyclophilin (CyP) of Gnathostoma spinigerum was cloned into a prokaryotic expression vector and expressed in Escherichia coli. The predicted molecular mass of the putative protein was 18.6. kDa, and the deduced amino acid sequence had 86, 84.8, 81.3 and 77.2% identity...

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Main Authors: Porntip Laummaunwai, Pewpan M. Intapan, Chaisiri Wongkham, Viraphong Lulitanond, Chatchai Tayapiwatana, Wanchai Maleewong
Format: Journal
Published: 2018
Subjects:
Immunology and Microbiology
Medicine
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80054913334&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50912
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-509122018-09-04T04:50:00Z Gnathostoma spinigerum: Molecular cloning, expression and characterization of the cyclophilin protein Porntip Laummaunwai Pewpan M. Intapan Chaisiri Wongkham Viraphong Lulitanond Chatchai Tayapiwatana Wanchai Maleewong Immunology and Microbiology Medicine In this study, a cDNA encoding cyclophilin (CyP) of Gnathostoma spinigerum was cloned into a prokaryotic expression vector and expressed in Escherichia coli. The predicted molecular mass of the putative protein was 18.6. kDa, and the deduced amino acid sequence had 86, 84.8, 81.3 and 77.2% identity with the CyP of Dirofilaria immitis, Brugia malayi, Onchocerca volvulus and Caenorhabditis elegans, respectively. A prediction of linear B-cell epitopes with high hydrophilicity and immunoblotting results indicated that the recombinant CyP has antigenicity to humans. The recombinant CyP protein reacted with human gnathostomiasis sera but not with other parasitosis or healthy control sera, suggesting that it might be useful for the serodiagnosis of human gnathostomiasis. © Elsevier Inc. 2018-09-04T04:47:26Z 2018-09-04T04:47:26Z 2010-12-01 Journal 10902449 00144894 2-s2.0-80054913334 10.1016/j.exppara.2010.06.004 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80054913334&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50912
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
Porntip Laummaunwai
Pewpan M. Intapan
Chaisiri Wongkham
Viraphong Lulitanond
Chatchai Tayapiwatana
Wanchai Maleewong
Gnathostoma spinigerum: Molecular cloning, expression and characterization of the cyclophilin protein
description In this study, a cDNA encoding cyclophilin (CyP) of Gnathostoma spinigerum was cloned into a prokaryotic expression vector and expressed in Escherichia coli. The predicted molecular mass of the putative protein was 18.6. kDa, and the deduced amino acid sequence had 86, 84.8, 81.3 and 77.2% identity with the CyP of Dirofilaria immitis, Brugia malayi, Onchocerca volvulus and Caenorhabditis elegans, respectively. A prediction of linear B-cell epitopes with high hydrophilicity and immunoblotting results indicated that the recombinant CyP has antigenicity to humans. The recombinant CyP protein reacted with human gnathostomiasis sera but not with other parasitosis or healthy control sera, suggesting that it might be useful for the serodiagnosis of human gnathostomiasis. © Elsevier Inc.
format Journal
author Porntip Laummaunwai
Pewpan M. Intapan
Chaisiri Wongkham
Viraphong Lulitanond
Chatchai Tayapiwatana
Wanchai Maleewong
author_facet Porntip Laummaunwai
Pewpan M. Intapan
Chaisiri Wongkham
Viraphong Lulitanond
Chatchai Tayapiwatana
Wanchai Maleewong
author_sort Porntip Laummaunwai
title Gnathostoma spinigerum: Molecular cloning, expression and characterization of the cyclophilin protein
title_short Gnathostoma spinigerum: Molecular cloning, expression and characterization of the cyclophilin protein
title_full Gnathostoma spinigerum: Molecular cloning, expression and characterization of the cyclophilin protein
title_fullStr Gnathostoma spinigerum: Molecular cloning, expression and characterization of the cyclophilin protein
title_full_unstemmed Gnathostoma spinigerum: Molecular cloning, expression and characterization of the cyclophilin protein
title_sort gnathostoma spinigerum: molecular cloning, expression and characterization of the cyclophilin protein
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80054913334&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50912
_version_ 1681423674995900416

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