Production of monoclonal antibodies to P-glycoprotein: Its application in detection of soluble and surface P-glycoprotein of leukemia patients

• Main Authors: Sawitree Chiampanichayakul, Songyot Anuchapreeda, Nuttapol Chruewkamlow, Kodchakorn Mahasongkram, Pattra Thanaratanakorn, Watchara Kasinrerk
• Format: Journal
• Published: 2018
• Subjects: Medicine
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77956896273&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/51044
• Institution: Chiang Mai University

Multidrug resistance (MDR) in leukemia is commonly associated with the expression of a transmembrane protein, P-glycoprotein (P-gp). In this study, two monoclonal antibodies (mAbs) specific for the extracellular domain of P-gp were generated. By employing the generated mAbs, a two-color lysed whole...