Comparison of glucose derivatives effects on cartilage degradation

Background. Glucosamine (GlcN) is a well-recognized candidate for treatment of osteoarthritis. However, it is currently used in derivative forms, such as glucosamine-hydrochloride (GlcN-HCl) or glucosamine sulfate (GlcN-S). However, the molecular mode of action remains unclear. In this study, we com...

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Main Authors: Thanyaluck Phitak, Peraphan Pothacharoen, Prachya Kongtawelert
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/51051
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-510512018-09-04T04:50:46Z Comparison of glucose derivatives effects on cartilage degradation Thanyaluck Phitak Peraphan Pothacharoen Prachya Kongtawelert Medicine Background. Glucosamine (GlcN) is a well-recognized candidate for treatment of osteoarthritis. However, it is currently used in derivative forms, such as glucosamine-hydrochloride (GlcN-HCl) or glucosamine sulfate (GlcN-S). However, the molecular mode of action remains unclear. In this study, we compared the effects of Glucose (Glc), Glucuronic acid (GlcA), Glucosamine hydrochloride (GlcN-HCl) and Glucosamine sulfate (GlcN-S) on cartilage degradation. Methods. Porcine cartilage explants were co-cultured with recombinant human IL-1β and each tested substance for 3 days. HA, s-GAG and MMP-2 releases to media were measured using ELISA, dye-binding assay and gelatin zymography, respectively. Similar studies were performed in a human articular chondrocytes (HAC) monolayer culture, where cells were co-treated with IL-1β and each reagent for 24 hours. Subsequently, cells were harvested and gene expression measured using RT-PCR. All experiments were carried out in triplicate. Student's t-tests were used for statistical analysis. Results. In cartilage explants treated with IL-1β, GlcN-S had the highest chondroprotective activity of all four chemicals as shown by the inhibition of HA, s-GAG and MMP-2 released from cartilage. The anabolic (aggrecan core protein; AGG, SOX9) and catabolic (MMP-3, -13) genes in HACs treated with IL-1β and with/without chemicals were studied using RT-PCR. It was found that, GlcN-HCl and GlcN-S could reduce the expression of both MMP-3 and -13 genes. The IL-1β induced-MMP-13 gene expression was decreased maximally by GlcN-S, while the reduction of induced-MMP-3 gene expression was greatest with GlcN-HCl. Glc and GlcA reversed the effect of IL-1β on the expression of AGG and SOX9, but other substances had no effect. Conclusion. This study shows that glucosamine derivatives can alter anabolic and catabolic processes in HACs induced by IL-1β. GlcN-S and GluN-HCl decreased induced MMP-3 and -13 expressions, while Glc and GlcA increased reduced-AGG and SOX9 expression. The chondroprotective study using porcine cartilage explant showed that GlcN-S had the strongest effect. © 2010 Phitak et al; licensee BioMed Central Ltd. 2018-09-04T04:50:46Z 2018-09-04T04:50:46Z 2010-07-19 Journal 14712474 2-s2.0-77954557733 10.1186/1471-2474-11-162 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77954557733&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51051
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Thanyaluck Phitak
Peraphan Pothacharoen
Prachya Kongtawelert
Comparison of glucose derivatives effects on cartilage degradation
description Background. Glucosamine (GlcN) is a well-recognized candidate for treatment of osteoarthritis. However, it is currently used in derivative forms, such as glucosamine-hydrochloride (GlcN-HCl) or glucosamine sulfate (GlcN-S). However, the molecular mode of action remains unclear. In this study, we compared the effects of Glucose (Glc), Glucuronic acid (GlcA), Glucosamine hydrochloride (GlcN-HCl) and Glucosamine sulfate (GlcN-S) on cartilage degradation. Methods. Porcine cartilage explants were co-cultured with recombinant human IL-1β and each tested substance for 3 days. HA, s-GAG and MMP-2 releases to media were measured using ELISA, dye-binding assay and gelatin zymography, respectively. Similar studies were performed in a human articular chondrocytes (HAC) monolayer culture, where cells were co-treated with IL-1β and each reagent for 24 hours. Subsequently, cells were harvested and gene expression measured using RT-PCR. All experiments were carried out in triplicate. Student's t-tests were used for statistical analysis. Results. In cartilage explants treated with IL-1β, GlcN-S had the highest chondroprotective activity of all four chemicals as shown by the inhibition of HA, s-GAG and MMP-2 released from cartilage. The anabolic (aggrecan core protein; AGG, SOX9) and catabolic (MMP-3, -13) genes in HACs treated with IL-1β and with/without chemicals were studied using RT-PCR. It was found that, GlcN-HCl and GlcN-S could reduce the expression of both MMP-3 and -13 genes. The IL-1β induced-MMP-13 gene expression was decreased maximally by GlcN-S, while the reduction of induced-MMP-3 gene expression was greatest with GlcN-HCl. Glc and GlcA reversed the effect of IL-1β on the expression of AGG and SOX9, but other substances had no effect. Conclusion. This study shows that glucosamine derivatives can alter anabolic and catabolic processes in HACs induced by IL-1β. GlcN-S and GluN-HCl decreased induced MMP-3 and -13 expressions, while Glc and GlcA increased reduced-AGG and SOX9 expression. The chondroprotective study using porcine cartilage explant showed that GlcN-S had the strongest effect. © 2010 Phitak et al; licensee BioMed Central Ltd.
format Journal
author Thanyaluck Phitak
Peraphan Pothacharoen
Prachya Kongtawelert
author_facet Thanyaluck Phitak
Peraphan Pothacharoen
Prachya Kongtawelert
author_sort Thanyaluck Phitak
title Comparison of glucose derivatives effects on cartilage degradation
title_short Comparison of glucose derivatives effects on cartilage degradation
title_full Comparison of glucose derivatives effects on cartilage degradation
title_fullStr Comparison of glucose derivatives effects on cartilage degradation
title_full_unstemmed Comparison of glucose derivatives effects on cartilage degradation
title_sort comparison of glucose derivatives effects on cartilage degradation
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77954557733&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51051
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