Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis

Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis

Molecular epidemiology analyses of the 36 clinical isolates of Pasteurella multocida from various avian hosts in Japan between 1976 to 2007 including 5 reference strains from the U.S.A., Taiwan and Indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (SE-AFL...

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Main Authors: Nattawooti Sthitmatee, Yasushi Kataoka, Takuo Sawada
Format: Journal
Published: 2018
Subjects:
Veterinary
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78649798054&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51183
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Institution: Chiang Mai University
id th-cmuir.6653943832-51183
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spelling th-cmuir.6653943832-511832018-09-04T04:54:20Z Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis Nattawooti Sthitmatee Yasushi Kataoka Takuo Sawada Veterinary Molecular epidemiology analyses of the 36 clinical isolates of Pasteurella multocida from various avian hosts in Japan between 1976 to 2007 including 5 reference strains from the U.S.A., Taiwan and Indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (SE-AFLP) comparison with the classical ApaI-based pulsed-field gel electrophoresis (PFGE). As the results, SE-AFLP gave 21 profiles while PFGE gave 20 profiles. The Simpson's index of diversity analysis indicated that SE-AFLP gave a high discrimination power than PFGE. This concluded that SE-AFLP is a higher discrimination power than PFGE to differentiate avian P. multocida isolates in Japan. In addition, the genetical profiles suggested that there is the evolution of somatic serotype 3 strain in the indigenous host of Japan. 2018-09-04T04:54:20Z 2018-09-04T04:54:20Z 2010-11-01 Journal 13477439 09167250 2-s2.0-78649798054 10.1292/jvms.10-0181 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78649798054&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51183
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Veterinary
spellingShingle Veterinary
Nattawooti Sthitmatee
Yasushi Kataoka
Takuo Sawada
Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis
description Molecular epidemiology analyses of the 36 clinical isolates of Pasteurella multocida from various avian hosts in Japan between 1976 to 2007 including 5 reference strains from the U.S.A., Taiwan and Indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (SE-AFLP) comparison with the classical ApaI-based pulsed-field gel electrophoresis (PFGE). As the results, SE-AFLP gave 21 profiles while PFGE gave 20 profiles. The Simpson's index of diversity analysis indicated that SE-AFLP gave a high discrimination power than PFGE. This concluded that SE-AFLP is a higher discrimination power than PFGE to differentiate avian P. multocida isolates in Japan. In addition, the genetical profiles suggested that there is the evolution of somatic serotype 3 strain in the indigenous host of Japan.
format Journal
author Nattawooti Sthitmatee
Yasushi Kataoka
Takuo Sawada
author_facet Nattawooti Sthitmatee
Yasushi Kataoka
Takuo Sawada
author_sort Nattawooti Sthitmatee
title Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis
title_short Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis
title_full Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis
title_fullStr Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis
title_full_unstemmed Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis
title_sort molecular epidemiology of japanese avian pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78649798054&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51183
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