Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)

Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)

Aerobic bacteria were isolated from the midgut of Apis cerana indica on five different media. The highest count (5.4 × 107 colony forming units (CFU/g)) was obtained on Eosin methylene blue (EMB) medium. Fifty-eight isolates were purified and identified using biochemical tests, and 16S rRNA gene seq...

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Main Authors: Terd Disayathanoowat, Mikio Yoshiyama, Kiyoshi Kimura, Panuwan Chantawannakul
Format: Journal
Published: 2018
Subjects:
Agricultural and Biological Sciences
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84867750488&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51239
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-512392018-09-04T05:59:23Z Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica) Terd Disayathanoowat Mikio Yoshiyama Kiyoshi Kimura Panuwan Chantawannakul Agricultural and Biological Sciences Aerobic bacteria were isolated from the midgut of Apis cerana indica on five different media. The highest count (5.4 × 107 colony forming units (CFU/g)) was obtained on Eosin methylene blue (EMB) medium. Fifty-eight isolates were purified and identified using biochemical tests, and 16S rRNA gene sequences were analysed. Two groups of Gamma-proteobacteria and Firmicutes were found. The most abundant species were Klebsiella pneumoniae, Enterobacter cloacae and K. oxytoca. To determine their role in nutrition, enzymes produced from the bacterial isolates were assayed. Protease and lipase activities were detected in some bacterial isolates. The assimilation of fourteen carbon substrates was studied. D-mannose and raffinose showed the highest utilization. Paenibacillus larvae, the cause of American foulbrood disease, was inhibited by some members of Klebsiella. 2018-09-04T05:59:23Z 2018-09-04T05:59:23Z 2012-10-01 Journal 20786913 00218839 2-s2.0-84867750488 10.3896/IBRA.1.51.4.04 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84867750488&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51239
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Terd Disayathanoowat
Mikio Yoshiyama
Kiyoshi Kimura
Panuwan Chantawannakul
Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
description Aerobic bacteria were isolated from the midgut of Apis cerana indica on five different media. The highest count (5.4 × 107 colony forming units (CFU/g)) was obtained on Eosin methylene blue (EMB) medium. Fifty-eight isolates were purified and identified using biochemical tests, and 16S rRNA gene sequences were analysed. Two groups of Gamma-proteobacteria and Firmicutes were found. The most abundant species were Klebsiella pneumoniae, Enterobacter cloacae and K. oxytoca. To determine their role in nutrition, enzymes produced from the bacterial isolates were assayed. Protease and lipase activities were detected in some bacterial isolates. The assimilation of fourteen carbon substrates was studied. D-mannose and raffinose showed the highest utilization. Paenibacillus larvae, the cause of American foulbrood disease, was inhibited by some members of Klebsiella.
format Journal
author Terd Disayathanoowat
Mikio Yoshiyama
Kiyoshi Kimura
Panuwan Chantawannakul
author_facet Terd Disayathanoowat
Mikio Yoshiyama
Kiyoshi Kimura
Panuwan Chantawannakul
author_sort Terd Disayathanoowat
title Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
title_short Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
title_full Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
title_fullStr Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
title_full_unstemmed Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
title_sort isolation and characterization of bacteria from the midgut of the asian honey bee (apis cerana indica)
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84867750488&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51239
_version_ 1681423733937405952

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