Preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin
This study aimed at developing a method for the preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin for the determination of insecticide residues in contaminated fruits and vegetables. 3-(2, 2-dichlorovinyl)-2, 2-dimethyl - (1-cyclopane) carboxylic acid (DC...
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th-cmuir.6653943832-512902018-09-04T05:59:55Z Preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin Surat Hongsibsong Jiraprapa Wipasa Mookda Pattarawarapan Prasak Thavornyutikarn Somporn Chantara Tippawan Prapamontol Agricultural and Biological Sciences This study aimed at developing a method for the preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin for the determination of insecticide residues in contaminated fruits and vegetables. 3-(2, 2-dichlorovinyl)-2, 2-dimethyl - (1-cyclopane) carboxylic acid (DCCA) was used as a spacer arm of hapten preparation. DCCA was linked to beta alanine methyl ester and conjugated with bovine serum albumin (BSA) as an immunogen and with oval albumin (OVA) as a coating antigen. BALB/c mice were immunized with immunogen. After three doses, sera from each mouse were collected and used to develop an ELISA. 0.05% Tween20/PBS with 20% methanol was used as diluents. The obtained polyclonal antibody (pAb) gave IC50 to cypermethrin at 5.2 μg/well. The lowest quantification limit of detection of the ELISA was 0.18 μg/well at 85% B/B0 and the detection limit was 0.039 μg/well at 90% B/B0. The antibody will be applicable for developing ELISA for screening of cypermethrin residue in vegetable and fruit samples and enable to save cost of currently used analytical procedures. © 2012 Friends Science Publishers. 2018-09-04T05:59:55Z 2018-09-04T05:59:55Z 2012-03-21 Journal 18149596 15608530 2-s2.0-84858314833 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84858314833&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51290 |
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Agricultural and Biological Sciences Surat Hongsibsong Jiraprapa Wipasa Mookda Pattarawarapan Prasak Thavornyutikarn Somporn Chantara Tippawan Prapamontol Preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin |
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This study aimed at developing a method for the preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin for the determination of insecticide residues in contaminated fruits and vegetables. 3-(2, 2-dichlorovinyl)-2, 2-dimethyl - (1-cyclopane) carboxylic acid (DCCA) was used as a spacer arm of hapten preparation. DCCA was linked to beta alanine methyl ester and conjugated with bovine serum albumin (BSA) as an immunogen and with oval albumin (OVA) as a coating antigen. BALB/c mice were immunized with immunogen. After three doses, sera from each mouse were collected and used to develop an ELISA. 0.05% Tween20/PBS with 20% methanol was used as diluents. The obtained polyclonal antibody (pAb) gave IC50 to cypermethrin at 5.2 μg/well. The lowest quantification limit of detection of the ELISA was 0.18 μg/well at 85% B/B0 and the detection limit was 0.039 μg/well at 90% B/B0. The antibody will be applicable for developing ELISA for screening of cypermethrin residue in vegetable and fruit samples and enable to save cost of currently used analytical procedures. © 2012 Friends Science Publishers. |
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Surat Hongsibsong Jiraprapa Wipasa Mookda Pattarawarapan Prasak Thavornyutikarn Somporn Chantara Tippawan Prapamontol |
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Surat Hongsibsong Jiraprapa Wipasa Mookda Pattarawarapan Prasak Thavornyutikarn Somporn Chantara Tippawan Prapamontol |
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Surat Hongsibsong |
title |
Preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin |
title_short |
Preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin |
title_full |
Preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin |
title_fullStr |
Preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin |
title_full_unstemmed |
Preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin |
title_sort |
preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84858314833&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51290 |
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