Rapid identification of heterozygous and homozygous Hemoglobin Constant Spring by SYTO9 with a high resolution melting analysis

Background: Gel-electrophoresis and ethidium bromide are not ideally suited to large scale analysis in clinical laboratories. Methods: Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) specific for Hb CS was performed in 10 blood samples from normal individuals and 61 sam...

Full description

Saved in:
Bibliographic Details
Main Authors: Sakorn Pornprasert, Sitthichai Panyasai, Jarurin Waneesorn, Kanyakan Kongthai, Panthong Singboottra
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84866135069&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51357
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-51357
record_format dspace
spelling th-cmuir.6653943832-513572018-09-04T06:00:44Z Rapid identification of heterozygous and homozygous Hemoglobin Constant Spring by SYTO9 with a high resolution melting analysis Sakorn Pornprasert Sitthichai Panyasai Jarurin Waneesorn Kanyakan Kongthai Panthong Singboottra Biochemistry, Genetics and Molecular Biology Background: Gel-electrophoresis and ethidium bromide are not ideally suited to large scale analysis in clinical laboratories. Methods: Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) specific for Hb CS was performed in 10 blood samples from normal individuals and 61 samples containing a peak of Hb CS when analyzed by capillary electrophoresis. Heterozygosity of Hb CS was identified using SYTO9 and high resolution melting (HRM) analysis method. Results: Specific peak heights of amplified fragments of wild type and Hb CS alleles were observed in the heterozygote. Only one peak height of amplified fragments of the wild type allele was observed in the normal individual while only one peak height of amplified fragments of Hb CS allele was observed in the homozygote. HRM analysis interpretation results were completely consistent with the interpretation results from gel-electrophoresis. Conclusions: SYTO9 HRM analysis may be used as an alternative for rapid diagnosis of heterozygosity of Hb CS. 2018-09-04T06:00:44Z 2018-09-04T06:00:44Z 2012-09-17 Journal 14336510 2-s2.0-84866135069 10.7754/Clin.Lab.2011.110903 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84866135069&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51357
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Sakorn Pornprasert
Sitthichai Panyasai
Jarurin Waneesorn
Kanyakan Kongthai
Panthong Singboottra
Rapid identification of heterozygous and homozygous Hemoglobin Constant Spring by SYTO9 with a high resolution melting analysis
description Background: Gel-electrophoresis and ethidium bromide are not ideally suited to large scale analysis in clinical laboratories. Methods: Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) specific for Hb CS was performed in 10 blood samples from normal individuals and 61 samples containing a peak of Hb CS when analyzed by capillary electrophoresis. Heterozygosity of Hb CS was identified using SYTO9 and high resolution melting (HRM) analysis method. Results: Specific peak heights of amplified fragments of wild type and Hb CS alleles were observed in the heterozygote. Only one peak height of amplified fragments of the wild type allele was observed in the normal individual while only one peak height of amplified fragments of Hb CS allele was observed in the homozygote. HRM analysis interpretation results were completely consistent with the interpretation results from gel-electrophoresis. Conclusions: SYTO9 HRM analysis may be used as an alternative for rapid diagnosis of heterozygosity of Hb CS.
format Journal
author Sakorn Pornprasert
Sitthichai Panyasai
Jarurin Waneesorn
Kanyakan Kongthai
Panthong Singboottra
author_facet Sakorn Pornprasert
Sitthichai Panyasai
Jarurin Waneesorn
Kanyakan Kongthai
Panthong Singboottra
author_sort Sakorn Pornprasert
title Rapid identification of heterozygous and homozygous Hemoglobin Constant Spring by SYTO9 with a high resolution melting analysis
title_short Rapid identification of heterozygous and homozygous Hemoglobin Constant Spring by SYTO9 with a high resolution melting analysis
title_full Rapid identification of heterozygous and homozygous Hemoglobin Constant Spring by SYTO9 with a high resolution melting analysis
title_fullStr Rapid identification of heterozygous and homozygous Hemoglobin Constant Spring by SYTO9 with a high resolution melting analysis
title_full_unstemmed Rapid identification of heterozygous and homozygous Hemoglobin Constant Spring by SYTO9 with a high resolution melting analysis
title_sort rapid identification of heterozygous and homozygous hemoglobin constant spring by syto9 with a high resolution melting analysis
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84866135069&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51357
_version_ 1681423755186798592