Evaluation and comparison of the efficiency of immunochromatography methods for norovirus detection
Background: The efficiency of a commercial immunochromatography kit (IP-NoV) was evaluated for rapid detection of norovirus in Japanese fecal specimens and its sensitivity and specificity were compared with two other commercial kits. Methods: A total of 70 samples collected from children who suffere...
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| Main Authors: | , , , , , , , |
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| Format: | Journal |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84863698008&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51370 |
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| Institution: | Chiang Mai University |
| Summary: | Background: The efficiency of a commercial immunochromatography kit (IP-NoV) was evaluated for rapid detection of norovirus in Japanese fecal specimens and its sensitivity and specificity were compared with two other commercial kits. Methods: A total of 70 samples collected from children who suffered from acute gastroenteritis in 2009-2010 were tested for norovirus by three different immunochromatography kits. RT-PCR was employed as the gold standard method. Results: The sensitivity of IP-NoV, QuickEx-Norovirus, and QuickNavi-Norovirus kits were 85.2%, 63.9%, and 55.7%, respectively. The IP-NoV kit could detect various genotypes of norovirus with higher efficiency as compared to QuickEx-Norovirus and QuickNavi-Norovirus kits. In addition, the phylogenetic analysis revealed that the majority of noroviruses circulating in Japan during 2009-2010 belonged to GII/4 variant 2006b and 2008a, which were responsible for the outbreaks worldwide. Conclusions: The findings indicated that the IC kits could be used as an alternative method for direct detection of norovirus in clinical specimens covering a wide range of norovirus genotypes circulating in Japan. |
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