Association and expression study of MMP3, TGFb1 and COL10A1 as candidate genes for leg weakness-related traits in pigs

The present study was aimed to determine the association between metalloproteinase 3 (MMP3), transforming growth factor beta 1 (TGFb1) and collagen type X alpha I (COL10A1) gene polymorphisms with traits related to leg weakness in pigs. Three hundred Duroc 9 Pietrain cross breds (DuPi) and 299 pigs...

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Main Authors: Watchara Laenoi, Noppawan Rangkasenee, Muhammad Jasim Uddin, Mehmet Ulas Cinar, Chirawath Phatsara, Dawit Tesfaye, Armin M. Scholz, Ernst Tholen, Christian Looft, Manfred Mielenz, Helga Sauerwein, Klaus Wimmers, Karl Schellander
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/51409
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spelling th-cmuir.6653943832-514092018-09-04T06:01:24Z Association and expression study of MMP3, TGFb1 and COL10A1 as candidate genes for leg weakness-related traits in pigs Watchara Laenoi Noppawan Rangkasenee Muhammad Jasim Uddin Mehmet Ulas Cinar Chirawath Phatsara Dawit Tesfaye Armin M. Scholz Ernst Tholen Christian Looft Manfred Mielenz Helga Sauerwein Klaus Wimmers Karl Schellander Biochemistry, Genetics and Molecular Biology The present study was aimed to determine the association between metalloproteinase 3 (MMP3), transforming growth factor beta 1 (TGFb1) and collagen type X alpha I (COL10A1) gene polymorphisms with traits related to leg weakness in pigs. Three hundred Duroc 9 Pietrain cross breds (DuPi) and 299 pigs of a commercial population (CP) were used for the experiment. DuPi animals were examined for 10 different traits describing leg and feet structure, osteochondrosis (OC) scores and bone density status. Data of OC score at condylus medialis humeri, condylus medialis femoris and distal epiphysis ulna regions of CP were used for association analysis. Significant association (P<0.05) was found for MMP3 SNP (g.158 C>T) with OC at head of femur and bone mineral density in the DuPi population. Association (P<0.05) was found between SNP of TGFb1 (g.180 G>A) with rear leg score and the principle component denoting both OC and feet and leg scores in the DuPi population. No association was found between COL10A1 (g.72 C>T) and leg weakness related traits. The associations of SNPs with OC traits could not be confirmed in the commercial population. Expression analysis of the three candidate genes was performed to compare between healthy and OC. TGFb1 was found to be highly expressed (P<0.05) in the OC compared to healthy cartilages, but no significant different expressions were observed for MMP3 and COL10A1 genes. The present finding suggested that TGFb1 and MMP3 genes variants have an effect on some of the leg weakness related traits. © Springer Science+Business Media B.V. 2011. 2018-09-04T06:01:24Z 2018-09-04T06:01:24Z 2012-04-01 Journal 15734978 03014851 2-s2.0-84862996889 10.1007/s11033-011-1168-5 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84862996889&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51409
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Watchara Laenoi
Noppawan Rangkasenee
Muhammad Jasim Uddin
Mehmet Ulas Cinar
Chirawath Phatsara
Dawit Tesfaye
Armin M. Scholz
Ernst Tholen
Christian Looft
Manfred Mielenz
Helga Sauerwein
Klaus Wimmers
Karl Schellander
Association and expression study of MMP3, TGFb1 and COL10A1 as candidate genes for leg weakness-related traits in pigs
description The present study was aimed to determine the association between metalloproteinase 3 (MMP3), transforming growth factor beta 1 (TGFb1) and collagen type X alpha I (COL10A1) gene polymorphisms with traits related to leg weakness in pigs. Three hundred Duroc 9 Pietrain cross breds (DuPi) and 299 pigs of a commercial population (CP) were used for the experiment. DuPi animals were examined for 10 different traits describing leg and feet structure, osteochondrosis (OC) scores and bone density status. Data of OC score at condylus medialis humeri, condylus medialis femoris and distal epiphysis ulna regions of CP were used for association analysis. Significant association (P<0.05) was found for MMP3 SNP (g.158 C>T) with OC at head of femur and bone mineral density in the DuPi population. Association (P<0.05) was found between SNP of TGFb1 (g.180 G>A) with rear leg score and the principle component denoting both OC and feet and leg scores in the DuPi population. No association was found between COL10A1 (g.72 C>T) and leg weakness related traits. The associations of SNPs with OC traits could not be confirmed in the commercial population. Expression analysis of the three candidate genes was performed to compare between healthy and OC. TGFb1 was found to be highly expressed (P<0.05) in the OC compared to healthy cartilages, but no significant different expressions were observed for MMP3 and COL10A1 genes. The present finding suggested that TGFb1 and MMP3 genes variants have an effect on some of the leg weakness related traits. © Springer Science+Business Media B.V. 2011.
format Journal
author Watchara Laenoi
Noppawan Rangkasenee
Muhammad Jasim Uddin
Mehmet Ulas Cinar
Chirawath Phatsara
Dawit Tesfaye
Armin M. Scholz
Ernst Tholen
Christian Looft
Manfred Mielenz
Helga Sauerwein
Klaus Wimmers
Karl Schellander
author_facet Watchara Laenoi
Noppawan Rangkasenee
Muhammad Jasim Uddin
Mehmet Ulas Cinar
Chirawath Phatsara
Dawit Tesfaye
Armin M. Scholz
Ernst Tholen
Christian Looft
Manfred Mielenz
Helga Sauerwein
Klaus Wimmers
Karl Schellander
author_sort Watchara Laenoi
title Association and expression study of MMP3, TGFb1 and COL10A1 as candidate genes for leg weakness-related traits in pigs
title_short Association and expression study of MMP3, TGFb1 and COL10A1 as candidate genes for leg weakness-related traits in pigs
title_full Association and expression study of MMP3, TGFb1 and COL10A1 as candidate genes for leg weakness-related traits in pigs
title_fullStr Association and expression study of MMP3, TGFb1 and COL10A1 as candidate genes for leg weakness-related traits in pigs
title_full_unstemmed Association and expression study of MMP3, TGFb1 and COL10A1 as candidate genes for leg weakness-related traits in pigs
title_sort association and expression study of mmp3, tgfb1 and col10a1 as candidate genes for leg weakness-related traits in pigs
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84862996889&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51409
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