Sugar ester synthesis by thermostable lipase from Streptomyces thermocarboxydus ME168

• Main Authors: Aran H-Kittikun, Poonsuk Prasertsan, Wolfgang Zimmermann, Phisit Seesuriyachan, Thanongsak Chaiyaso
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Immunology and Microbiology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84860833634&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/51411
• Institution: Chiang Mai University

The extracellular lipase from Streptomyces thermocarboxydus ME168 was purified to 9.5-fold with 20% yield, following concentration by acetone precipitation, ion exchange chromatography (Resource Q) and gel filtration chromatography (Superdex 200), respectively. The purified enzyme had an apparent mo...