Development of control material for hemoglobin analysis

Background: There is no certified control material for hemoglobin analysis which has the hemoglobin (Hb)A2/E level as high as found in β-thalassemia trait, HbE trait, β-thalassemia/HbE disease and homozygote of HbE, the thalassemia types found frequently in the Southeast Asian population. The aim of...

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Main Authors: Sakorn Pornprasert, Kunyakan Kongthai, Jarurin Waneesorn, Kanokwan Jaiping, Kallayanee Treesuwan
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/51415
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-514152018-09-04T06:12:06Z Development of control material for hemoglobin analysis Sakorn Pornprasert Kunyakan Kongthai Jarurin Waneesorn Kanokwan Jaiping Kallayanee Treesuwan Biochemistry, Genetics and Molecular Biology Medicine Background: There is no certified control material for hemoglobin analysis which has the hemoglobin (Hb)A2/E level as high as found in β-thalassemia trait, HbE trait, β-thalassemia/HbE disease and homozygote of HbE, the thalassemia types found frequently in the Southeast Asian population. The aim of this study was to prepare the lyophilized hemoglobin control materials for hemoglobin analysis. Methods: Washed and dialysed erythrocytes of normal individuals and patients with β-thalassemia trait, HbE trait, β-thalassemia/HbE disease, homozygous HbE were lysed in 5 sucrose solution. The lyophilized hemoglobin control materials were prepared by using a freeze-drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized hemoglobin was performed after storing at 20°C for 1, 15 and 30days and for 3months. Results: The chromatograms of lyophilized hemoglobin control materials showed similar patterns and similar levels of HbA, HbA2/E and HbF when compared with equivalent fresh whole blood. Moreover, the lyophilized hemoglobin presented a good correlation coefficient (r>0.990) of relationships between HPLC, low pressure liquid chromatography (LPLC) and capillary electrophoresis (CE) methods. Conclusions: The lyophilized hemoglobin could be developed and used as control materials for hemoglobin analysis. © 2012 by Walter de Gruyter Berlin Boston. 2018-09-04T06:01:36Z 2018-09-04T06:01:36Z 2012-02-01 Journal 14374331 14346621 2-s2.0-84860517682 10.1515/cclm.2011.777 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84860517682&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51415
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Sakorn Pornprasert
Kunyakan Kongthai
Jarurin Waneesorn
Kanokwan Jaiping
Kallayanee Treesuwan
Development of control material for hemoglobin analysis
description Background: There is no certified control material for hemoglobin analysis which has the hemoglobin (Hb)A2/E level as high as found in β-thalassemia trait, HbE trait, β-thalassemia/HbE disease and homozygote of HbE, the thalassemia types found frequently in the Southeast Asian population. The aim of this study was to prepare the lyophilized hemoglobin control materials for hemoglobin analysis. Methods: Washed and dialysed erythrocytes of normal individuals and patients with β-thalassemia trait, HbE trait, β-thalassemia/HbE disease, homozygous HbE were lysed in 5 sucrose solution. The lyophilized hemoglobin control materials were prepared by using a freeze-drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized hemoglobin was performed after storing at 20°C for 1, 15 and 30days and for 3months. Results: The chromatograms of lyophilized hemoglobin control materials showed similar patterns and similar levels of HbA, HbA2/E and HbF when compared with equivalent fresh whole blood. Moreover, the lyophilized hemoglobin presented a good correlation coefficient (r>0.990) of relationships between HPLC, low pressure liquid chromatography (LPLC) and capillary electrophoresis (CE) methods. Conclusions: The lyophilized hemoglobin could be developed and used as control materials for hemoglobin analysis. © 2012 by Walter de Gruyter Berlin Boston.
format Journal
author Sakorn Pornprasert
Kunyakan Kongthai
Jarurin Waneesorn
Kanokwan Jaiping
Kallayanee Treesuwan
author_facet Sakorn Pornprasert
Kunyakan Kongthai
Jarurin Waneesorn
Kanokwan Jaiping
Kallayanee Treesuwan
author_sort Sakorn Pornprasert
title Development of control material for hemoglobin analysis
title_short Development of control material for hemoglobin analysis
title_full Development of control material for hemoglobin analysis
title_fullStr Development of control material for hemoglobin analysis
title_full_unstemmed Development of control material for hemoglobin analysis
title_sort development of control material for hemoglobin analysis
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84860517682&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51415
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