Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging

Leaves of Longkong which collected from Chantaburi in Thailand were extracted by the hot and cold processes using three different solvents including water, chloroform and methanol. The crude extracts were tested for antioxidative activities, tyrosinase inhibition and in vitro cytotoxicity as well as...

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Main Authors: Aranya Manosroi, Kulthida Kumguan, Charinya Chankhampan, Worapaka Manosroi, Jiradej Manosroi
Format: Journal
Published: 2018
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-514502018-09-04T06:14:01Z Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging Aranya Manosroi Kulthida Kumguan Charinya Chankhampan Worapaka Manosroi Jiradej Manosroi Chemical Engineering Chemistry Engineering Materials Science Physics and Astronomy Leaves of Longkong which collected from Chantaburi in Thailand were extracted by the hot and cold processes using three different solvents including water, chloroform and methanol. The crude extracts were tested for antioxidative activities, tyrosinase inhibition and in vitro cytotoxicity as well as the MMP-2 inhibition activity on human skin fibroblasts for anti-aging evaluation. The hot water crude extract showed the highest antioxidative activities (DPPH radical scavenging, metal ion chelating and lipid peroxidation inhibition) with the SC50, CC50and IPC50values of 5.40±1.23, 32.31±0.84 and 3.29±0.30 mg/ml, respectively, and the highest tyrosinase inhibition activity with the IC50value of 0.49±0.23 mg/ml. The extract also showed no cytotoxicity on human skin fibroblasts with the cell viability of 80.52±15.16%. It demonstrated the anti-aging potential by having the pro and active MMP-2 inhibition activity, but lower than ascorbic acid of 1.28 and 1.12 times, respectively. The semi-purified extracts were prepared from this crude extract by solvent-solvent partition. The ethyl acetate soluble fraction showed higher activities (DPPH radical scavenging, metal ion chelating and tyrosinase inhibition) than the crude extract of 23.48, 71.80 and 2.58 times, respectively. This fraction exhibited similar pro and active MMP-2 inhibitory effect to the crude extract. The results from this study have indicated the possible application of the ethyl acetate fraction of the hot water crude extract from leaves of Longkong to be developed as an anti-aging product. Copyright © 2012 American Scientific Publishers All rights reserved. 2018-09-04T06:02:06Z 2018-09-04T06:02:06Z 2012-09-01 Journal 15334899 15334880 2-s2.0-84866084536 10.1166/jnn.2012.6500 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84866084536&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51450
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemical Engineering
Chemistry
Engineering
Materials Science
Physics and Astronomy
spellingShingle Chemical Engineering
Chemistry
Engineering
Materials Science
Physics and Astronomy
Aranya Manosroi
Kulthida Kumguan
Charinya Chankhampan
Worapaka Manosroi
Jiradej Manosroi
Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
description Leaves of Longkong which collected from Chantaburi in Thailand were extracted by the hot and cold processes using three different solvents including water, chloroform and methanol. The crude extracts were tested for antioxidative activities, tyrosinase inhibition and in vitro cytotoxicity as well as the MMP-2 inhibition activity on human skin fibroblasts for anti-aging evaluation. The hot water crude extract showed the highest antioxidative activities (DPPH radical scavenging, metal ion chelating and lipid peroxidation inhibition) with the SC50, CC50and IPC50values of 5.40±1.23, 32.31±0.84 and 3.29±0.30 mg/ml, respectively, and the highest tyrosinase inhibition activity with the IC50value of 0.49±0.23 mg/ml. The extract also showed no cytotoxicity on human skin fibroblasts with the cell viability of 80.52±15.16%. It demonstrated the anti-aging potential by having the pro and active MMP-2 inhibition activity, but lower than ascorbic acid of 1.28 and 1.12 times, respectively. The semi-purified extracts were prepared from this crude extract by solvent-solvent partition. The ethyl acetate soluble fraction showed higher activities (DPPH radical scavenging, metal ion chelating and tyrosinase inhibition) than the crude extract of 23.48, 71.80 and 2.58 times, respectively. This fraction exhibited similar pro and active MMP-2 inhibitory effect to the crude extract. The results from this study have indicated the possible application of the ethyl acetate fraction of the hot water crude extract from leaves of Longkong to be developed as an anti-aging product. Copyright © 2012 American Scientific Publishers All rights reserved.
format Journal
author Aranya Manosroi
Kulthida Kumguan
Charinya Chankhampan
Worapaka Manosroi
Jiradej Manosroi
author_facet Aranya Manosroi
Kulthida Kumguan
Charinya Chankhampan
Worapaka Manosroi
Jiradej Manosroi
author_sort Aranya Manosroi
title Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
title_short Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
title_full Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
title_fullStr Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
title_full_unstemmed Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
title_sort nanoscale gelatinase a (mmp-2) inhibition on human skin fibroblasts of longkong (lansium domesticum correa) leaf extracts for anti-aging
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84866084536&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51450
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