Sequential injection spectrophotometric system for evaluation of mushroom tyrosinase-inhibitory activity

A sequential injection (SI) spectrophotometric method with absorbance detection at 475 nm has been developed for evaluating activity of some compounds on an inhibition of the mushroom tyrosinase. The method involved a reaction of 3,4-dihydroxyphenylalanine (l-DOPA) and mushroom tyrosinase to form th...

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Main Authors: Nuntaporn Moonrungsee, Tomoko Shimamura, Takehiro Kashiwagi, Jaroon Jakmunee, Keiro Higuchi, Hiroyuki Ukeda
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/51471
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-514712018-09-04T06:02:30Z Sequential injection spectrophotometric system for evaluation of mushroom tyrosinase-inhibitory activity Nuntaporn Moonrungsee Tomoko Shimamura Takehiro Kashiwagi Jaroon Jakmunee Keiro Higuchi Hiroyuki Ukeda Chemistry A sequential injection (SI) spectrophotometric method with absorbance detection at 475 nm has been developed for evaluating activity of some compounds on an inhibition of the mushroom tyrosinase. The method involved a reaction of 3,4-dihydroxyphenylalanine (l-DOPA) and mushroom tyrosinase to form the o-dopaquinone. The decrease of the o-dopaquinone was related to an increase of tyrosinase-inhibitory activity. Under the optimum conditions (concentration and volume of l-DOPA and mushroom tyrosinase of 2.0 mM, 60 μL and 142 U mL-1, 15 μL, respectively), some antioxidant compounds were examined for the tyrosinase-inhibitory activity. A batch enzymatic assay of tyrosinase-inhibitory activity was applied as the reference method for comparison. The results of IC50values obtained from the proposed method and the batch method were correlated well, with r2of 0.969. The SIA provides higher precision and degrees of automation, consumes smaller amounts of chemicals and it is simpler and faster than the batch method. © 2012 Elsevier B.V. All rights reserved. 2018-09-04T06:02:30Z 2018-09-04T06:02:30Z 2012-11-15 Journal 00399140 2-s2.0-84869878836 10.1016/j.talanta.2012.09.015 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84869878836&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51471
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
spellingShingle Chemistry
Nuntaporn Moonrungsee
Tomoko Shimamura
Takehiro Kashiwagi
Jaroon Jakmunee
Keiro Higuchi
Hiroyuki Ukeda
Sequential injection spectrophotometric system for evaluation of mushroom tyrosinase-inhibitory activity
description A sequential injection (SI) spectrophotometric method with absorbance detection at 475 nm has been developed for evaluating activity of some compounds on an inhibition of the mushroom tyrosinase. The method involved a reaction of 3,4-dihydroxyphenylalanine (l-DOPA) and mushroom tyrosinase to form the o-dopaquinone. The decrease of the o-dopaquinone was related to an increase of tyrosinase-inhibitory activity. Under the optimum conditions (concentration and volume of l-DOPA and mushroom tyrosinase of 2.0 mM, 60 μL and 142 U mL-1, 15 μL, respectively), some antioxidant compounds were examined for the tyrosinase-inhibitory activity. A batch enzymatic assay of tyrosinase-inhibitory activity was applied as the reference method for comparison. The results of IC50values obtained from the proposed method and the batch method were correlated well, with r2of 0.969. The SIA provides higher precision and degrees of automation, consumes smaller amounts of chemicals and it is simpler and faster than the batch method. © 2012 Elsevier B.V. All rights reserved.
format Journal
author Nuntaporn Moonrungsee
Tomoko Shimamura
Takehiro Kashiwagi
Jaroon Jakmunee
Keiro Higuchi
Hiroyuki Ukeda
author_facet Nuntaporn Moonrungsee
Tomoko Shimamura
Takehiro Kashiwagi
Jaroon Jakmunee
Keiro Higuchi
Hiroyuki Ukeda
author_sort Nuntaporn Moonrungsee
title Sequential injection spectrophotometric system for evaluation of mushroom tyrosinase-inhibitory activity
title_short Sequential injection spectrophotometric system for evaluation of mushroom tyrosinase-inhibitory activity
title_full Sequential injection spectrophotometric system for evaluation of mushroom tyrosinase-inhibitory activity
title_fullStr Sequential injection spectrophotometric system for evaluation of mushroom tyrosinase-inhibitory activity
title_full_unstemmed Sequential injection spectrophotometric system for evaluation of mushroom tyrosinase-inhibitory activity
title_sort sequential injection spectrophotometric system for evaluation of mushroom tyrosinase-inhibitory activity
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84869878836&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51471
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