Genotoxicity and antigenotoxicity of the methanol extract of Cleistocalyx nervosum var. paniala seed using a Salmonella mutation assay and rat liver micronucleus tests

Clesitocalyx nervosum var. paniala, an edible fruit found in some parts of Southeast Asia including Thailand, contains high amounts of polyphenols and has multiple biological activities. The purposes of this study were to evaluate the genotoxic and antigenotoxic effects of methanol extracts of C. ne...

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Main Authors: Wanida Inboot, Sirinya Taya, Anuruk Chailungka, Puttinan Meepowpan, Rawiwan Wongpoomchai
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/51692
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spelling th-cmuir.6653943832-516922018-09-04T06:13:26Z Genotoxicity and antigenotoxicity of the methanol extract of Cleistocalyx nervosum var. paniala seed using a Salmonella mutation assay and rat liver micronucleus tests Wanida Inboot Sirinya Taya Anuruk Chailungka Puttinan Meepowpan Rawiwan Wongpoomchai Environmental Science Medicine Pharmacology, Toxicology and Pharmaceutics Clesitocalyx nervosum var. paniala, an edible fruit found in some parts of Southeast Asia including Thailand, contains high amounts of polyphenols and has multiple biological activities. The purposes of this study were to evaluate the genotoxic and antigenotoxic effects of methanol extracts of C. nervosum seeds via a Salmonella mutation assay and a rat liver micronucleus test. C. nervosum extract was not mutagenic to Salmonella typhimurium strains TA98 and TA100 in both the presence and absence of metabolic activation. Furthermore, C. nervosum seed extract presented antigenotoxicity against aflatoxin B1, MeIQ and AF-2 induced mutagenesis. Clastogenicity and anticlastogenicity of C. nervosum seed extracts were determined in rat livers. Male wistar rats were divided into 6 groups. Groups 1 and 3 were treated with 5% tween-80 as a vehicle control. Group 2 received 1,000 mg/kg bw of methanol seed extract and groups 4-6 were fed with 20, 100 and 1,000 mg/kg bw of seed extracts, respectively for 21 days. At day 15 and 18 of the experiment, treated rats in groups 3-6 were intraperitoneally injected with 30 mg/kg bw of diethylnitrosamine to initiate hepatocarcinogenesis. At day 22, all rats were partially hepatectomized to amplify mutated hepatocytes. C. nervosum seed extract did not affect micronucleus formation in rat livers, but did slightly decrease the frequencies of micronucleated hepatocytes of diethylnitrosamine treated rats. In conclusion, the methanol extract of C. nervosum seed may contain chemopreventive compounds against carcinogenesis. © 2012 The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Netherlands. 2018-09-04T06:06:28Z 2018-09-04T06:06:28Z 2012-03-01 Journal 20928467 1738642X 2-s2.0-84859557324 10.1007/s13273-012-0003-y https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859557324&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51692
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Environmental Science
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Environmental Science
Medicine
Pharmacology, Toxicology and Pharmaceutics
Wanida Inboot
Sirinya Taya
Anuruk Chailungka
Puttinan Meepowpan
Rawiwan Wongpoomchai
Genotoxicity and antigenotoxicity of the methanol extract of Cleistocalyx nervosum var. paniala seed using a Salmonella mutation assay and rat liver micronucleus tests
description Clesitocalyx nervosum var. paniala, an edible fruit found in some parts of Southeast Asia including Thailand, contains high amounts of polyphenols and has multiple biological activities. The purposes of this study were to evaluate the genotoxic and antigenotoxic effects of methanol extracts of C. nervosum seeds via a Salmonella mutation assay and a rat liver micronucleus test. C. nervosum extract was not mutagenic to Salmonella typhimurium strains TA98 and TA100 in both the presence and absence of metabolic activation. Furthermore, C. nervosum seed extract presented antigenotoxicity against aflatoxin B1, MeIQ and AF-2 induced mutagenesis. Clastogenicity and anticlastogenicity of C. nervosum seed extracts were determined in rat livers. Male wistar rats were divided into 6 groups. Groups 1 and 3 were treated with 5% tween-80 as a vehicle control. Group 2 received 1,000 mg/kg bw of methanol seed extract and groups 4-6 were fed with 20, 100 and 1,000 mg/kg bw of seed extracts, respectively for 21 days. At day 15 and 18 of the experiment, treated rats in groups 3-6 were intraperitoneally injected with 30 mg/kg bw of diethylnitrosamine to initiate hepatocarcinogenesis. At day 22, all rats were partially hepatectomized to amplify mutated hepatocytes. C. nervosum seed extract did not affect micronucleus formation in rat livers, but did slightly decrease the frequencies of micronucleated hepatocytes of diethylnitrosamine treated rats. In conclusion, the methanol extract of C. nervosum seed may contain chemopreventive compounds against carcinogenesis. © 2012 The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Netherlands.
format Journal
author Wanida Inboot
Sirinya Taya
Anuruk Chailungka
Puttinan Meepowpan
Rawiwan Wongpoomchai
author_facet Wanida Inboot
Sirinya Taya
Anuruk Chailungka
Puttinan Meepowpan
Rawiwan Wongpoomchai
author_sort Wanida Inboot
title Genotoxicity and antigenotoxicity of the methanol extract of Cleistocalyx nervosum var. paniala seed using a Salmonella mutation assay and rat liver micronucleus tests
title_short Genotoxicity and antigenotoxicity of the methanol extract of Cleistocalyx nervosum var. paniala seed using a Salmonella mutation assay and rat liver micronucleus tests
title_full Genotoxicity and antigenotoxicity of the methanol extract of Cleistocalyx nervosum var. paniala seed using a Salmonella mutation assay and rat liver micronucleus tests
title_fullStr Genotoxicity and antigenotoxicity of the methanol extract of Cleistocalyx nervosum var. paniala seed using a Salmonella mutation assay and rat liver micronucleus tests
title_full_unstemmed Genotoxicity and antigenotoxicity of the methanol extract of Cleistocalyx nervosum var. paniala seed using a Salmonella mutation assay and rat liver micronucleus tests
title_sort genotoxicity and antigenotoxicity of the methanol extract of cleistocalyx nervosum var. paniala seed using a salmonella mutation assay and rat liver micronucleus tests
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859557324&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51692
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