Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand

Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand

Twenty bacterial strains isolated from the blood of patients with suspected Streptococcus suis infection based on clinical symptoms in northern Thailand between 2009 and 2010 were subjected to phenotypic and genotypic identification. Commercial identification kits and a PCR-based assay targeting the...

Full description

Saved in:
Bibliographic Details
Main Authors: Le Hong Thuy Tien, Naoya Sugiyama, Kwanjit Duangsonk, Prasit Tharavichitkul, Ro Osawa
Format: Journal
Published: 2018
Subjects:
Medicine
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859018828&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51908
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-51908
record_format dspace
spelling th-cmuir.6653943832-519082018-09-04T06:11:31Z Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand Le Hong Thuy Tien Naoya Sugiyama Kwanjit Duangsonk Prasit Tharavichitkul Ro Osawa Medicine Twenty bacterial strains isolated from the blood of patients with suspected Streptococcus suis infection based on clinical symptoms in northern Thailand between 2009 and 2010 were subjected to phenotypic and genotypic identification. Commercial identification kits and a PCR-based assay targeting the S. suis-specific 16S rDNA sequence correctly identified S. suis isolated from patients in northern Thailand; however, there was a risk of misidentifying S. gallolyticus as S. suis using a PCR assay targeting the S. suis-specific house keeping gene encoding glutamate dehydrogenase. This is the first paper to report S. gallolyticus infection in humans in Thailand. 2018-09-04T06:11:31Z 2018-09-04T06:11:31Z 2012-04-02 Journal 18842836 13446304 2-s2.0-84859018828 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859018828&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51908
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Le Hong Thuy Tien
Naoya Sugiyama
Kwanjit Duangsonk
Prasit Tharavichitkul
Ro Osawa
Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
description Twenty bacterial strains isolated from the blood of patients with suspected Streptococcus suis infection based on clinical symptoms in northern Thailand between 2009 and 2010 were subjected to phenotypic and genotypic identification. Commercial identification kits and a PCR-based assay targeting the S. suis-specific 16S rDNA sequence correctly identified S. suis isolated from patients in northern Thailand; however, there was a risk of misidentifying S. gallolyticus as S. suis using a PCR assay targeting the S. suis-specific house keeping gene encoding glutamate dehydrogenase. This is the first paper to report S. gallolyticus infection in humans in Thailand.
format Journal
author Le Hong Thuy Tien
Naoya Sugiyama
Kwanjit Duangsonk
Prasit Tharavichitkul
Ro Osawa
author_facet Le Hong Thuy Tien
Naoya Sugiyama
Kwanjit Duangsonk
Prasit Tharavichitkul
Ro Osawa
author_sort Le Hong Thuy Tien
title Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
title_short Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
title_full Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
title_fullStr Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
title_full_unstemmed Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
title_sort phenotypic and pcr-based identification of bacterial strains isolated from patients with suspected streptococcus suis infection in northern thailand
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859018828&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51908
_version_ 1681423854730215424

Similar Items