Low-energy ion beam bombardment effect on the plant-cell-envelope mimetic membrane for DNA transfer

Low-energy ion beam bombardment effect on the plant-cell-envelope mimetic membrane for DNA transfer

This study is a systematic analysis of the mechanisms involved in ion-beam induced DNA transfer, an important application of ion beam biotechnology. Cellulose membranes were used to mimic the plant cell envelope. Ion beams of argon (Ar) or nitrogen (N) at an energy of 25 keV bombarded the cellulose...

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Main Authors: K. Prakrajang, K. Sangwijit, S. Anuntalabhochai, P. Wanichapichart, L. D. Yu
Format: Journal
Published: 2018
Subjects:
Physics and Astronomy
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84865461448&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51987
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-519872018-09-04T06:14:04Z Low-energy ion beam bombardment effect on the plant-cell-envelope mimetic membrane for DNA transfer K. Prakrajang K. Sangwijit S. Anuntalabhochai P. Wanichapichart L. D. Yu Physics and Astronomy This study is a systematic analysis of the mechanisms involved in ion-beam induced DNA transfer, an important application of ion beam biotechnology. Cellulose membranes were used to mimic the plant cell envelope. Ion beams of argon (Ar) or nitrogen (N) at an energy of 25 keV bombarded the cellulose membranes at fluences ranging from 1015to 1016ions/cm2. The damage to the ion-beam-bombarded membranes was characterized using infrared spectroscopy, a micro tensile test and scanning electron microscopy (SEM). Chain scission was the dominant radiation damage type in the membrane. DNA diffusion across the membrane was significantly increased after ion beam bombardment. The increase in DNA transfer is therefore attributed to chain scission, which increases the permeability by increasing the number of pores in the membrane. © 2011 Elsevier B.V. All rights reserved. 2018-09-04T06:14:04Z 2018-09-04T06:14:04Z 2012-09-01 Journal 0168583X 2-s2.0-84865461448 10.1016/j.nimb.2012.02.025 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84865461448&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51987
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Physics and Astronomy
spellingShingle Physics and Astronomy
K. Prakrajang
K. Sangwijit
S. Anuntalabhochai
P. Wanichapichart
L. D. Yu
Low-energy ion beam bombardment effect on the plant-cell-envelope mimetic membrane for DNA transfer
description This study is a systematic analysis of the mechanisms involved in ion-beam induced DNA transfer, an important application of ion beam biotechnology. Cellulose membranes were used to mimic the plant cell envelope. Ion beams of argon (Ar) or nitrogen (N) at an energy of 25 keV bombarded the cellulose membranes at fluences ranging from 1015to 1016ions/cm2. The damage to the ion-beam-bombarded membranes was characterized using infrared spectroscopy, a micro tensile test and scanning electron microscopy (SEM). Chain scission was the dominant radiation damage type in the membrane. DNA diffusion across the membrane was significantly increased after ion beam bombardment. The increase in DNA transfer is therefore attributed to chain scission, which increases the permeability by increasing the number of pores in the membrane. © 2011 Elsevier B.V. All rights reserved.
format Journal
author K. Prakrajang
K. Sangwijit
S. Anuntalabhochai
P. Wanichapichart
L. D. Yu
author_facet K. Prakrajang
K. Sangwijit
S. Anuntalabhochai
P. Wanichapichart
L. D. Yu
author_sort K. Prakrajang
title Low-energy ion beam bombardment effect on the plant-cell-envelope mimetic membrane for DNA transfer
title_short Low-energy ion beam bombardment effect on the plant-cell-envelope mimetic membrane for DNA transfer
title_full Low-energy ion beam bombardment effect on the plant-cell-envelope mimetic membrane for DNA transfer
title_fullStr Low-energy ion beam bombardment effect on the plant-cell-envelope mimetic membrane for DNA transfer
title_full_unstemmed Low-energy ion beam bombardment effect on the plant-cell-envelope mimetic membrane for DNA transfer
title_sort low-energy ion beam bombardment effect on the plant-cell-envelope mimetic membrane for dna transfer
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84865461448&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51987
_version_ 1681423868998189056

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