Hybrid detection in lychee (Litchee chinensis Sonn.) cultivars using HAT-RAPD markers

In this paper we present a method to detect hybrids obtained from open-pollinated seeds in lychee cultivars (Litchi chinensis Sonn.) using the high annealing temperature randomly amplified polymorphic DNA (HAT-RAPD) methodology. A series of 10 arbitrary random primers were used to amplify polymorphi...

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Main Authors: Chundet R., Cutler R.W., Tasanon M., Anuntalabhochai S.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-34948848554&partnerID=40&md5=93aa09f65eec8a333418f111199bf73a
http://cmuir.cmu.ac.th/handle/6653943832/5200
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-52002014-08-30T02:56:15Z Hybrid detection in lychee (Litchee chinensis Sonn.) cultivars using HAT-RAPD markers Chundet R. Cutler R.W. Tasanon M. Anuntalabhochai S. In this paper we present a method to detect hybrids obtained from open-pollinated seeds in lychee cultivars (Litchi chinensis Sonn.) using the high annealing temperature randomly amplified polymorphic DNA (HAT-RAPD) methodology. A series of 10 arbitrary random primers were used to amplify polymorphic DNA bands ranging from 200 to 1,700 bp. These bands were tracked from parent to hybrid offspring for crosses of four economically important lychee varieties (Chacrapat, Kimjeng, Hong Huey and O-Hia) and both gender and variety specific band transmission rates were assessed. Irrespective of lychee variety, all hybrids showed a significant loss of parental bands with the largest losses occurring between the male parent and the offspring. Variety-specific bands were characterized for each lychee variety and hybrid band transmission rates were determined such that hybrids could be detected to any level of desired significance given enough initial banding data. This molecular marker technique provides a clear and compelling way to ascertain hybrid status of new plants without having to wait the six to seven years for the plants to mature. 2014-08-30T02:56:15Z 2014-08-30T02:56:15Z 2007 Article 15131874 10.2306/scienceasia1513-1874.2007.33.307 http://www.scopus.com/inward/record.url?eid=2-s2.0-34948848554&partnerID=40&md5=93aa09f65eec8a333418f111199bf73a http://cmuir.cmu.ac.th/handle/6653943832/5200 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description In this paper we present a method to detect hybrids obtained from open-pollinated seeds in lychee cultivars (Litchi chinensis Sonn.) using the high annealing temperature randomly amplified polymorphic DNA (HAT-RAPD) methodology. A series of 10 arbitrary random primers were used to amplify polymorphic DNA bands ranging from 200 to 1,700 bp. These bands were tracked from parent to hybrid offspring for crosses of four economically important lychee varieties (Chacrapat, Kimjeng, Hong Huey and O-Hia) and both gender and variety specific band transmission rates were assessed. Irrespective of lychee variety, all hybrids showed a significant loss of parental bands with the largest losses occurring between the male parent and the offspring. Variety-specific bands were characterized for each lychee variety and hybrid band transmission rates were determined such that hybrids could be detected to any level of desired significance given enough initial banding data. This molecular marker technique provides a clear and compelling way to ascertain hybrid status of new plants without having to wait the six to seven years for the plants to mature.
format Article
author Chundet R.
Cutler R.W.
Tasanon M.
Anuntalabhochai S.
spellingShingle Chundet R.
Cutler R.W.
Tasanon M.
Anuntalabhochai S.
Hybrid detection in lychee (Litchee chinensis Sonn.) cultivars using HAT-RAPD markers
author_facet Chundet R.
Cutler R.W.
Tasanon M.
Anuntalabhochai S.
author_sort Chundet R.
title Hybrid detection in lychee (Litchee chinensis Sonn.) cultivars using HAT-RAPD markers
title_short Hybrid detection in lychee (Litchee chinensis Sonn.) cultivars using HAT-RAPD markers
title_full Hybrid detection in lychee (Litchee chinensis Sonn.) cultivars using HAT-RAPD markers
title_fullStr Hybrid detection in lychee (Litchee chinensis Sonn.) cultivars using HAT-RAPD markers
title_full_unstemmed Hybrid detection in lychee (Litchee chinensis Sonn.) cultivars using HAT-RAPD markers
title_sort hybrid detection in lychee (litchee chinensis sonn.) cultivars using hat-rapd markers
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-34948848554&partnerID=40&md5=93aa09f65eec8a333418f111199bf73a
http://cmuir.cmu.ac.th/handle/6653943832/5200
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