A rapid survey technique for tropilaelaps mite (Mesostigmata: Laelapidae) detection

Parasitic Tropilaelaps (Delfinado and Baker) mites are a damaging pest of European honey bees (Apis mellifera L.) in Asia. These mites represent a significant threat if introduced to other regions of the world, warranting implementation of Tropilaelaps mite surveillance in uninfested regions. Curren...

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Main Authors: Jeffery S. Pettis, Robyn Rose, Elinor M. Lichtenberg, Panuwan Chantawannakul, Ninat Buawangpong, Weeraya Somana, Prachaval Sukumalanand, Dennis Vanengelsdorp
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/52089
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spelling th-cmuir.6653943832-520892018-09-04T09:27:52Z A rapid survey technique for tropilaelaps mite (Mesostigmata: Laelapidae) detection Jeffery S. Pettis Robyn Rose Elinor M. Lichtenberg Panuwan Chantawannakul Ninat Buawangpong Weeraya Somana Prachaval Sukumalanand Dennis Vanengelsdorp Agricultural and Biological Sciences Environmental Science Parasitic Tropilaelaps (Delfinado and Baker) mites are a damaging pest of European honey bees (Apis mellifera L.) in Asia. These mites represent a significant threat if introduced to other regions of the world, warranting implementation of Tropilaelaps mite surveillance in uninfested regions. Current Tropilaelaps mite-detection methods are unsuitable for efficient large scale screening. We developed and tested a new bump technique that consists of firmly rapping a honey bee brood frame over a collecting pan. Our method was easier to implement than current detection tests, reduced time spent in each apiary, and minimized brood destruction. This feasibility increase overcomes the test's decreased rate of detecting infested colonies (sensitivity; 36.3% for the bump test, 54.2% and 56.7% for the two most sensitive methods currently used in Asia). Considering this sensitivity, we suggest that screening programs sample seven colonies per apiary (independent of apiary size) and 312 randomly selected apiaries in a region to be 95% sure of detecting an incipient Tropilaelaps mite invasion. Further analyses counter the currently held view that Tropilaelaps mites prefer drone bee brood cells. Tropilaelaps mite infestation rate was 3.5 ± 0.9% in drone brood and 5.7 ± 0.6% in worker brood. We propose the bump test as a standard tool for monitoring of Tropilaelaps mite presence in regions thought to be free from infestation. However, regulators may favor the sensitivity of the Drop test (collecting mites that fall to the bottom of a hive on sticky boards) over the less time-intensive Bump test. 2018-09-04T09:20:53Z 2018-09-04T09:20:53Z 2013-08-01 Journal 00220493 2-s2.0-84881638909 10.1603/EC12339 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84881638909&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52089
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Environmental Science
spellingShingle Agricultural and Biological Sciences
Environmental Science
Jeffery S. Pettis
Robyn Rose
Elinor M. Lichtenberg
Panuwan Chantawannakul
Ninat Buawangpong
Weeraya Somana
Prachaval Sukumalanand
Dennis Vanengelsdorp
A rapid survey technique for tropilaelaps mite (Mesostigmata: Laelapidae) detection
description Parasitic Tropilaelaps (Delfinado and Baker) mites are a damaging pest of European honey bees (Apis mellifera L.) in Asia. These mites represent a significant threat if introduced to other regions of the world, warranting implementation of Tropilaelaps mite surveillance in uninfested regions. Current Tropilaelaps mite-detection methods are unsuitable for efficient large scale screening. We developed and tested a new bump technique that consists of firmly rapping a honey bee brood frame over a collecting pan. Our method was easier to implement than current detection tests, reduced time spent in each apiary, and minimized brood destruction. This feasibility increase overcomes the test's decreased rate of detecting infested colonies (sensitivity; 36.3% for the bump test, 54.2% and 56.7% for the two most sensitive methods currently used in Asia). Considering this sensitivity, we suggest that screening programs sample seven colonies per apiary (independent of apiary size) and 312 randomly selected apiaries in a region to be 95% sure of detecting an incipient Tropilaelaps mite invasion. Further analyses counter the currently held view that Tropilaelaps mites prefer drone bee brood cells. Tropilaelaps mite infestation rate was 3.5 ± 0.9% in drone brood and 5.7 ± 0.6% in worker brood. We propose the bump test as a standard tool for monitoring of Tropilaelaps mite presence in regions thought to be free from infestation. However, regulators may favor the sensitivity of the Drop test (collecting mites that fall to the bottom of a hive on sticky boards) over the less time-intensive Bump test.
format Journal
author Jeffery S. Pettis
Robyn Rose
Elinor M. Lichtenberg
Panuwan Chantawannakul
Ninat Buawangpong
Weeraya Somana
Prachaval Sukumalanand
Dennis Vanengelsdorp
author_facet Jeffery S. Pettis
Robyn Rose
Elinor M. Lichtenberg
Panuwan Chantawannakul
Ninat Buawangpong
Weeraya Somana
Prachaval Sukumalanand
Dennis Vanengelsdorp
author_sort Jeffery S. Pettis
title A rapid survey technique for tropilaelaps mite (Mesostigmata: Laelapidae) detection
title_short A rapid survey technique for tropilaelaps mite (Mesostigmata: Laelapidae) detection
title_full A rapid survey technique for tropilaelaps mite (Mesostigmata: Laelapidae) detection
title_fullStr A rapid survey technique for tropilaelaps mite (Mesostigmata: Laelapidae) detection
title_full_unstemmed A rapid survey technique for tropilaelaps mite (Mesostigmata: Laelapidae) detection
title_sort rapid survey technique for tropilaelaps mite (mesostigmata: laelapidae) detection
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84881638909&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52089
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