Proteomic analysis of candidate prognostic urinary marker for cervical cancer

Urinary proteins are an important component in urine samples that can be used as biological indicators for clinical diagnosis of diseases, such as proteinuria, kidney failure, diseases in the bladder, urinary tract and related organs. In this present work, we investigated candidate protein biomarker...

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Main Authors: Paitoon Aobchey, Hataichanoke Niamsup, Sitthicha Siriaree, Boonyaras Sookkheo, Kongsak Boonyapranai, Shui Tein Chen
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/52168
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-521682018-09-04T09:25:02Z Proteomic analysis of candidate prognostic urinary marker for cervical cancer Paitoon Aobchey Hataichanoke Niamsup Sitthicha Siriaree Boonyaras Sookkheo Kongsak Boonyapranai Shui Tein Chen Biochemistry, Genetics and Molecular Biology Computer Science Urinary proteins are an important component in urine samples that can be used as biological indicators for clinical diagnosis of diseases, such as proteinuria, kidney failure, diseases in the bladder, urinary tract and related organs. In this present work, we investigated candidate protein biomarkers in urine for diagnosis of cervical cancer using proteomic approach. The urine samples were concentrated and de-salted through ultrafiltration (3 kDa), followed by Spin-50 mini-sephadex column and were then separated by two-dimensional electrophoresis (2-DE) and annotated by mass spectrometer. Comparing 2-DE results between individual healthy donors and cervical cancer, patients showed that 4 urinary proteins; Protocadherin-8, Aryl hydrocarbon receptor nuclear translocatorlike protein 2, Serum albumin and Endorepellin, C-terminal domain V of perlecan were overexpressed by two-fold in the patient samples. Only the endorepellin LG3 fragment was validated by Western blot analysis. We also used 2D Western blot and nano LC-MS/MS to confirm the urinary biomarker as endorepellin LG3 fragment. Our study suggests that proteomics approach may be useful for the study of urinary proteins and provides an opportunity to discovery aberrant protein-associated diseases. We also revealed the candidate biomarkers that may be applied for cervical cancer diagnosis which benefits for medical treatment. © 2013 Aobchey P, et al. 2018-09-04T09:21:43Z 2018-09-04T09:21:43Z 2013-12-12 Journal 0974276X 2-s2.0-84889667725 10.4172/jpb.1000287 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84889667725&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52168
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Computer Science
spellingShingle Biochemistry, Genetics and Molecular Biology
Computer Science
Paitoon Aobchey
Hataichanoke Niamsup
Sitthicha Siriaree
Boonyaras Sookkheo
Kongsak Boonyapranai
Shui Tein Chen
Proteomic analysis of candidate prognostic urinary marker for cervical cancer
description Urinary proteins are an important component in urine samples that can be used as biological indicators for clinical diagnosis of diseases, such as proteinuria, kidney failure, diseases in the bladder, urinary tract and related organs. In this present work, we investigated candidate protein biomarkers in urine for diagnosis of cervical cancer using proteomic approach. The urine samples were concentrated and de-salted through ultrafiltration (3 kDa), followed by Spin-50 mini-sephadex column and were then separated by two-dimensional electrophoresis (2-DE) and annotated by mass spectrometer. Comparing 2-DE results between individual healthy donors and cervical cancer, patients showed that 4 urinary proteins; Protocadherin-8, Aryl hydrocarbon receptor nuclear translocatorlike protein 2, Serum albumin and Endorepellin, C-terminal domain V of perlecan were overexpressed by two-fold in the patient samples. Only the endorepellin LG3 fragment was validated by Western blot analysis. We also used 2D Western blot and nano LC-MS/MS to confirm the urinary biomarker as endorepellin LG3 fragment. Our study suggests that proteomics approach may be useful for the study of urinary proteins and provides an opportunity to discovery aberrant protein-associated diseases. We also revealed the candidate biomarkers that may be applied for cervical cancer diagnosis which benefits for medical treatment. © 2013 Aobchey P, et al.
format Journal
author Paitoon Aobchey
Hataichanoke Niamsup
Sitthicha Siriaree
Boonyaras Sookkheo
Kongsak Boonyapranai
Shui Tein Chen
author_facet Paitoon Aobchey
Hataichanoke Niamsup
Sitthicha Siriaree
Boonyaras Sookkheo
Kongsak Boonyapranai
Shui Tein Chen
author_sort Paitoon Aobchey
title Proteomic analysis of candidate prognostic urinary marker for cervical cancer
title_short Proteomic analysis of candidate prognostic urinary marker for cervical cancer
title_full Proteomic analysis of candidate prognostic urinary marker for cervical cancer
title_fullStr Proteomic analysis of candidate prognostic urinary marker for cervical cancer
title_full_unstemmed Proteomic analysis of candidate prognostic urinary marker for cervical cancer
title_sort proteomic analysis of candidate prognostic urinary marker for cervical cancer
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84889667725&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52168
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