Criteria for detection of α-Thalassemia-1 Thai type deletion in routine laboratory
Background: Using a single tube multiplex real-time PCR with SYBR Greenl and high resolution melting (HRM) analysis in routine laboratory for detection of α-thalassemia-1 Southeast Asian (SEA) type deletion simultaneously with Thai type deletion is costly. Methods: Criteria for laboratory testing of...
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| Main Authors: | , , |
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| Format: | Journal |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84891600436&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52173 |
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| Institution: | Chiang Mai University |
| Summary: | Background: Using a single tube multiplex real-time PCR with SYBR Greenl and high resolution melting (HRM) analysis in routine laboratory for detection of α-thalassemia-1 Southeast Asian (SEA) type deletion simultaneously with Thai type deletion is costly. Methods: Criteria for laboratory testing of α-thalassemia-1 Thai type deletion were set as follow: (1) MCV ≤ 75 fL and negative for α-thalassemia-1 SEA type deletion, β-thalassemia or homozygous HbE; (2) HbE trait with HbE level < 21.5% and negative for α-thalassemia-1 SEA type deletion; or (3) Hb Bart's and HbH found on HPLC chromatogram but negative for α-thalassemia-1 SEA type deletion. Results: From May 2010 to October 2012, the number of samples to detect for α-thalassemia-1 Thai type deletion decreased from 15,081 to 672 samples and the α-thalassemia-1 Thai type deletion trait was found in 7 samples (1.04%). Conclusions: The new testing algorithm can reduce number of samples for detection of α-thalassemia-1 Thai type deletion. |
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