Detection of Hb H disease genotypes common in Northern Thailand by quantitative real-time polymerase chain reaction and high resolution melting analyses

• Main Authors: Teerapat Seeratanachot, Torpong Sanguansermsri, Dawan Shimbhu
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Medicine
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84887215064&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/52193
• Institution: Chiang Mai University

We used quantitative real-time polymerase chain reaction (qPCR) and high resolution melting (HRM) analyses for the detection of the common α-thalassemia (α-thal) genotypes in 40 northern Thai Hb H (β4) patients. The α0-thal [-SEA(Southeast Asian) deletion] was detected by a multiplex gap real-time P...