Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines
In-capillary derivatization and field-amplified sample injection (FASI) coupled to capillary zone electrophoresis (CZE) was evaluated for the analysis of metals (Co(II), Cu(II), Ni(II), and Fe(II)) using 2-(5-Nitro-2-Pyridylazo)-5-(N-Propyl-N-Sulfopropylamino)Phenol (Nitro-PAPS) as the derivatizing...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
2014
|
Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-34249321217&partnerID=40&md5=85549c4b3db694c851ba85facf8454b4 http://cmuir.cmu.ac.th/handle/6653943832/5221 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
Language: | English |
id |
th-cmuir.6653943832-5221 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-52212014-08-30T02:56:17Z Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines Santalad A. Burakham R. Srijaranai S. Grudpan K. In-capillary derivatization and field-amplified sample injection (FASI) coupled to capillary zone electrophoresis (CZE) was evaluated for the analysis of metals (Co(II), Cu(II), Ni(II), and Fe(II)) using 2-(5-Nitro-2-Pyridylazo)-5-(N-Propyl-N-Sulfopropylamino)Phenol (Nitro-PAPS) as the derivatizing agent. For FASI, the optimum conditions were water as sample solvent, 1 s hydrodynamic injection (0.1 psi) of a water plug, 5 s of electrokinetic introduction (10 kV) of the sample. The in-capillary derivatization was successfully achieved with zone-passing strategy in order tandem injection of Nitro-PAPS reagent (0.5 psi, 7 s), a small water plug (0.1 psi, 1 s), and metal ion introduction (10 kV, 5 s). The solution of 45 mmol L- 1 borate pH 9.7 and 1.0 × 10- 5 mol L- 1 Nitro-PAPS containing 20% acetonitrile was used as the running buffer. The limit of detection obtained by the proposed method was lower than those from pre-capillary derivatization about 3-28 times. The recovery of the method was comparable to pre-capillary derivatization method. In-capillary derivatization-FASI-CZE was applied to analysis of metals in wine samples. The results were compared with those obtained by CZE with pre-capillary derivatization method and atomic absorption spectrometry (AAS). © 2007 Elsevier B.V. All rights reserved. 2014-08-30T02:56:17Z 2014-08-30T02:56:17Z 2007 Article 0026265X 10.1016/j.microc.2007.03.006 MICJA http://www.scopus.com/inward/record.url?eid=2-s2.0-34249321217&partnerID=40&md5=85549c4b3db694c851ba85facf8454b4 http://cmuir.cmu.ac.th/handle/6653943832/5221 English |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
language |
English |
description |
In-capillary derivatization and field-amplified sample injection (FASI) coupled to capillary zone electrophoresis (CZE) was evaluated for the analysis of metals (Co(II), Cu(II), Ni(II), and Fe(II)) using 2-(5-Nitro-2-Pyridylazo)-5-(N-Propyl-N-Sulfopropylamino)Phenol (Nitro-PAPS) as the derivatizing agent. For FASI, the optimum conditions were water as sample solvent, 1 s hydrodynamic injection (0.1 psi) of a water plug, 5 s of electrokinetic introduction (10 kV) of the sample. The in-capillary derivatization was successfully achieved with zone-passing strategy in order tandem injection of Nitro-PAPS reagent (0.5 psi, 7 s), a small water plug (0.1 psi, 1 s), and metal ion introduction (10 kV, 5 s). The solution of 45 mmol L- 1 borate pH 9.7 and 1.0 × 10- 5 mol L- 1 Nitro-PAPS containing 20% acetonitrile was used as the running buffer. The limit of detection obtained by the proposed method was lower than those from pre-capillary derivatization about 3-28 times. The recovery of the method was comparable to pre-capillary derivatization method. In-capillary derivatization-FASI-CZE was applied to analysis of metals in wine samples. The results were compared with those obtained by CZE with pre-capillary derivatization method and atomic absorption spectrometry (AAS). © 2007 Elsevier B.V. All rights reserved. |
format |
Article |
author |
Santalad A. Burakham R. Srijaranai S. Grudpan K. |
spellingShingle |
Santalad A. Burakham R. Srijaranai S. Grudpan K. Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines |
author_facet |
Santalad A. Burakham R. Srijaranai S. Grudpan K. |
author_sort |
Santalad A. |
title |
Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines |
title_short |
Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines |
title_full |
Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines |
title_fullStr |
Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines |
title_full_unstemmed |
Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines |
title_sort |
field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines |
publishDate |
2014 |
url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-34249321217&partnerID=40&md5=85549c4b3db694c851ba85facf8454b4 http://cmuir.cmu.ac.th/handle/6653943832/5221 |
_version_ |
1681420384327434240 |