Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines

In-capillary derivatization and field-amplified sample injection (FASI) coupled to capillary zone electrophoresis (CZE) was evaluated for the analysis of metals (Co(II), Cu(II), Ni(II), and Fe(II)) using 2-(5-Nitro-2-Pyridylazo)-5-(N-Propyl-N-Sulfopropylamino)Phenol (Nitro-PAPS) as the derivatizing...

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Main Authors: Santalad A., Burakham R., Srijaranai S., Grudpan K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-34249321217&partnerID=40&md5=85549c4b3db694c851ba85facf8454b4
http://cmuir.cmu.ac.th/handle/6653943832/5221
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-52212014-08-30T02:56:17Z Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines Santalad A. Burakham R. Srijaranai S. Grudpan K. In-capillary derivatization and field-amplified sample injection (FASI) coupled to capillary zone electrophoresis (CZE) was evaluated for the analysis of metals (Co(II), Cu(II), Ni(II), and Fe(II)) using 2-(5-Nitro-2-Pyridylazo)-5-(N-Propyl-N-Sulfopropylamino)Phenol (Nitro-PAPS) as the derivatizing agent. For FASI, the optimum conditions were water as sample solvent, 1 s hydrodynamic injection (0.1 psi) of a water plug, 5 s of electrokinetic introduction (10 kV) of the sample. The in-capillary derivatization was successfully achieved with zone-passing strategy in order tandem injection of Nitro-PAPS reagent (0.5 psi, 7 s), a small water plug (0.1 psi, 1 s), and metal ion introduction (10 kV, 5 s). The solution of 45 mmol L- 1 borate pH 9.7 and 1.0 × 10- 5 mol L- 1 Nitro-PAPS containing 20% acetonitrile was used as the running buffer. The limit of detection obtained by the proposed method was lower than those from pre-capillary derivatization about 3-28 times. The recovery of the method was comparable to pre-capillary derivatization method. In-capillary derivatization-FASI-CZE was applied to analysis of metals in wine samples. The results were compared with those obtained by CZE with pre-capillary derivatization method and atomic absorption spectrometry (AAS). © 2007 Elsevier B.V. All rights reserved. 2014-08-30T02:56:17Z 2014-08-30T02:56:17Z 2007 Article 0026265X 10.1016/j.microc.2007.03.006 MICJA http://www.scopus.com/inward/record.url?eid=2-s2.0-34249321217&partnerID=40&md5=85549c4b3db694c851ba85facf8454b4 http://cmuir.cmu.ac.th/handle/6653943832/5221 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description In-capillary derivatization and field-amplified sample injection (FASI) coupled to capillary zone electrophoresis (CZE) was evaluated for the analysis of metals (Co(II), Cu(II), Ni(II), and Fe(II)) using 2-(5-Nitro-2-Pyridylazo)-5-(N-Propyl-N-Sulfopropylamino)Phenol (Nitro-PAPS) as the derivatizing agent. For FASI, the optimum conditions were water as sample solvent, 1 s hydrodynamic injection (0.1 psi) of a water plug, 5 s of electrokinetic introduction (10 kV) of the sample. The in-capillary derivatization was successfully achieved with zone-passing strategy in order tandem injection of Nitro-PAPS reagent (0.5 psi, 7 s), a small water plug (0.1 psi, 1 s), and metal ion introduction (10 kV, 5 s). The solution of 45 mmol L- 1 borate pH 9.7 and 1.0 × 10- 5 mol L- 1 Nitro-PAPS containing 20% acetonitrile was used as the running buffer. The limit of detection obtained by the proposed method was lower than those from pre-capillary derivatization about 3-28 times. The recovery of the method was comparable to pre-capillary derivatization method. In-capillary derivatization-FASI-CZE was applied to analysis of metals in wine samples. The results were compared with those obtained by CZE with pre-capillary derivatization method and atomic absorption spectrometry (AAS). © 2007 Elsevier B.V. All rights reserved.
format Article
author Santalad A.
Burakham R.
Srijaranai S.
Grudpan K.
spellingShingle Santalad A.
Burakham R.
Srijaranai S.
Grudpan K.
Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines
author_facet Santalad A.
Burakham R.
Srijaranai S.
Grudpan K.
author_sort Santalad A.
title Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines
title_short Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines
title_full Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines
title_fullStr Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines
title_full_unstemmed Field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines
title_sort field-amplified sample injection and in-capillary derivatization for capillary electrophoretic analysis of metal ions in local wines
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-34249321217&partnerID=40&md5=85549c4b3db694c851ba85facf8454b4
http://cmuir.cmu.ac.th/handle/6653943832/5221
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