Effects of BA and NAA on micropropagation and Stemona alkaloids production of Stemona curtisii Hook.f.

In vitro propagation of Stemona curtisii was explored for multiple shoot induction and root regeneration in different media. Shoot explants were cultured on solid MS media supplemented with 0, 1, 2 and 3 mg L-1benzyladenine (BA) to produce multiple shoots. This research revealed that the medium cont...

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Main Authors: Jiraporn Palee, Srisulak Dheeranupattana, Araya Jatisatienr, Sunanta Wangkarn
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/52214
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-522142018-09-04T09:36:32Z Effects of BA and NAA on micropropagation and Stemona alkaloids production of Stemona curtisii Hook.f. Jiraporn Palee Srisulak Dheeranupattana Araya Jatisatienr Sunanta Wangkarn Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy In vitro propagation of Stemona curtisii was explored for multiple shoot induction and root regeneration in different media. Shoot explants were cultured on solid MS media supplemented with 0, 1, 2 and 3 mg L-1benzyladenine (BA) to produce multiple shoots. This research revealed that the medium containing 2 mg L-1BA induced shoot formation at a rate of 100% with the highest number of 9.8 shoots per explant. Then, the elongated shoots were separated and cultured on MS solid and in liquid media supplemented with 0, 1, 2, 3 and 4 mg L-1naphthalene acetic acid (NAA) to induce roots. It was found that the solid medium containing 1 mg L-1NAA induced 100% root formation with the highest number of 21.5 roots per explant. For the Stemona alkaloids analysis from the in vitro roots, HPLC analysis showed the content of oxyprotostemonine 90 μg g-1DW, stemocurtisine 7 μg g-1DW and stemocurtisinol 9 μg g-1DW. 2018-09-04T09:22:14Z 2018-09-04T09:22:14Z 2013-08-06 Journal 01252526 2-s2.0-84880940806 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880940806&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52214
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
Jiraporn Palee
Srisulak Dheeranupattana
Araya Jatisatienr
Sunanta Wangkarn
Effects of BA and NAA on micropropagation and Stemona alkaloids production of Stemona curtisii Hook.f.
description In vitro propagation of Stemona curtisii was explored for multiple shoot induction and root regeneration in different media. Shoot explants were cultured on solid MS media supplemented with 0, 1, 2 and 3 mg L-1benzyladenine (BA) to produce multiple shoots. This research revealed that the medium containing 2 mg L-1BA induced shoot formation at a rate of 100% with the highest number of 9.8 shoots per explant. Then, the elongated shoots were separated and cultured on MS solid and in liquid media supplemented with 0, 1, 2, 3 and 4 mg L-1naphthalene acetic acid (NAA) to induce roots. It was found that the solid medium containing 1 mg L-1NAA induced 100% root formation with the highest number of 21.5 roots per explant. For the Stemona alkaloids analysis from the in vitro roots, HPLC analysis showed the content of oxyprotostemonine 90 μg g-1DW, stemocurtisine 7 μg g-1DW and stemocurtisinol 9 μg g-1DW.
format Journal
author Jiraporn Palee
Srisulak Dheeranupattana
Araya Jatisatienr
Sunanta Wangkarn
author_facet Jiraporn Palee
Srisulak Dheeranupattana
Araya Jatisatienr
Sunanta Wangkarn
author_sort Jiraporn Palee
title Effects of BA and NAA on micropropagation and Stemona alkaloids production of Stemona curtisii Hook.f.
title_short Effects of BA and NAA on micropropagation and Stemona alkaloids production of Stemona curtisii Hook.f.
title_full Effects of BA and NAA on micropropagation and Stemona alkaloids production of Stemona curtisii Hook.f.
title_fullStr Effects of BA and NAA on micropropagation and Stemona alkaloids production of Stemona curtisii Hook.f.
title_full_unstemmed Effects of BA and NAA on micropropagation and Stemona alkaloids production of Stemona curtisii Hook.f.
title_sort effects of ba and naa on micropropagation and stemona alkaloids production of stemona curtisii hook.f.
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880940806&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52214
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