Micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products

A simple micellar liquid chromatographic (MLC) procedure for simultaneous determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products was proposed. This method was developed and validated. The chromatographic conditions were also optimized. All analyses wer...

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Main Authors: W. Thogchai, B. Liawruangrath
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/52237
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spelling th-cmuir.6653943832-522372018-09-04T09:35:47Z Micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products W. Thogchai B. Liawruangrath Biochemistry, Genetics and Molecular Biology Chemical Engineering Chemistry Medicine Pharmacology, Toxicology and Pharmaceutics A simple micellar liquid chromatographic (MLC) procedure for simultaneous determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products was proposed. This method was developed and validated. The chromatographic conditions were also optimized. All analyses were performed at room temperature in an isocratic mode, using a mixture of 1% (v/v) acetonitrile and 0.006 mol L-1 Brij 35 (pH 6.0) as a mobile phase. The flow rate was set at 1.0 mL min-1. The analytical column was a 150 × 3.9 mm Nova-Pak C-18 column. The effluent from the analytical column was monitored by UV detection at 280 nm. Under the optimum conditions, arbutin and hydroquinone could be determined within a concentration range of 2-50 μg mL-1 of arbutin, and hydroquinone was obtained with the regression equations; y = 0.045x + 0.042 (r2 = 0.9923) and y = 0.091x + 0.050 (r2 = 0.9930) respectively. The limits of detection were found to be 0.51 μg mL-1 and 0.37 μg mL-1 for arbutin and hydroquinone respectively. The proposed MLC method was applied for the determination of arbutin and hydroquinone contents in medicinal plant extracts and commercial cosmetic products. This proposed MLC method is thus suitable for routine analysis of arbutin and hydroquinone in the pharmaceutical formulations, cosmetic products and raw medicinal plant extracts. © ICS © 2013 Society of Cosmetic Scientists and the Société Française de Cosmétologie. 2018-09-04T09:22:33Z 2018-09-04T09:22:33Z 2013-06-01 Journal 14682494 01425463 2-s2.0-84877827753 10.1111/ics.12037 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877827753&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52237
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Chemistry
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Chemistry
Medicine
Pharmacology, Toxicology and Pharmaceutics
W. Thogchai
B. Liawruangrath
Micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products
description A simple micellar liquid chromatographic (MLC) procedure for simultaneous determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products was proposed. This method was developed and validated. The chromatographic conditions were also optimized. All analyses were performed at room temperature in an isocratic mode, using a mixture of 1% (v/v) acetonitrile and 0.006 mol L-1 Brij 35 (pH 6.0) as a mobile phase. The flow rate was set at 1.0 mL min-1. The analytical column was a 150 × 3.9 mm Nova-Pak C-18 column. The effluent from the analytical column was monitored by UV detection at 280 nm. Under the optimum conditions, arbutin and hydroquinone could be determined within a concentration range of 2-50 μg mL-1 of arbutin, and hydroquinone was obtained with the regression equations; y = 0.045x + 0.042 (r2 = 0.9923) and y = 0.091x + 0.050 (r2 = 0.9930) respectively. The limits of detection were found to be 0.51 μg mL-1 and 0.37 μg mL-1 for arbutin and hydroquinone respectively. The proposed MLC method was applied for the determination of arbutin and hydroquinone contents in medicinal plant extracts and commercial cosmetic products. This proposed MLC method is thus suitable for routine analysis of arbutin and hydroquinone in the pharmaceutical formulations, cosmetic products and raw medicinal plant extracts. © ICS © 2013 Society of Cosmetic Scientists and the Société Française de Cosmétologie.
format Journal
author W. Thogchai
B. Liawruangrath
author_facet W. Thogchai
B. Liawruangrath
author_sort W. Thogchai
title Micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products
title_short Micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products
title_full Micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products
title_fullStr Micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products
title_full_unstemmed Micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products
title_sort micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877827753&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52237
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