Transdermal absorption and stability enhancement of salmon calcitonin by Tat peptide

Context: Highly organized structure of stratum corneum (SC) is the major barrier of the delivery of macromolecules such as proteins and peptides across the skin. Recently, cell penetrating peptides (CPPs) such as HIV1-trans-activating transcriptional (Tat) have been used to enhance the topical deliv...

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Main Authors: Jiradej Manosroi, Warangkana Lohcharoenkal, Friedrich Götz, Rolf G. Werner, Worapaka Manosroi, Aranya Manosroi
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/52401
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-524012018-09-04T09:35:51Z Transdermal absorption and stability enhancement of salmon calcitonin by Tat peptide Jiradej Manosroi Warangkana Lohcharoenkal Friedrich Götz Rolf G. Werner Worapaka Manosroi Aranya Manosroi Chemistry Medicine Pharmacology, Toxicology and Pharmaceutics Context: Highly organized structure of stratum corneum (SC) is the major barrier of the delivery of macromolecules such as proteins and peptides across the skin. Recently, cell penetrating peptides (CPPs) such as HIV1-trans-activating transcriptional (Tat) have been used to enhance the topical delivery of proteins and peptides. Objective: This study aimed to enhance the transdermal absorption and chemical stability of salmon calcitonin (sCT) by co-incubation with Tat. Materials and methods: Tat-sCT mixture at 1:1 molar ratio was prepared. Transdermal absorption and chemical stability of the mixture was evaluated in comparing with free sCT. Results: Tat-sCT mixture gave higher cumulative amounts and fluxes of sCT than free sCT. The maximum percentage of sCT of 58.36 ± 12.33% permeated into the receiving chamber was found in Tat-sCT mixture at 6 h which was 3.50 folds of free sCT. Tat-sCT mixture demonstrated better sCT stability than sCT solution after 1 month storage at 4°C, 25°C and 45°C. Discussion: The positively-charged arginine groups in Tat might be responsible for the binding of peptide complexes to negatively charged cell surfaces by electrostatic interactions and also the translocation of sCT through the excised skin. Conclusion: This study demonstrated the enhancements of transdermal absorption and stability of sCT by Tat peptide with potential for further application in transdermal delivery of other therapeutic peptides. © 2013 Informa Healthcare USA, Inc. 2018-09-04T09:24:47Z 2018-09-04T09:24:47Z 2013-04-01 Journal 15205762 03639045 2-s2.0-84874503901 10.3109/03639045.2012.684388 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84874503901&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52401
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Chemistry
Medicine
Pharmacology, Toxicology and Pharmaceutics
Jiradej Manosroi
Warangkana Lohcharoenkal
Friedrich Götz
Rolf G. Werner
Worapaka Manosroi
Aranya Manosroi
Transdermal absorption and stability enhancement of salmon calcitonin by Tat peptide
description Context: Highly organized structure of stratum corneum (SC) is the major barrier of the delivery of macromolecules such as proteins and peptides across the skin. Recently, cell penetrating peptides (CPPs) such as HIV1-trans-activating transcriptional (Tat) have been used to enhance the topical delivery of proteins and peptides. Objective: This study aimed to enhance the transdermal absorption and chemical stability of salmon calcitonin (sCT) by co-incubation with Tat. Materials and methods: Tat-sCT mixture at 1:1 molar ratio was prepared. Transdermal absorption and chemical stability of the mixture was evaluated in comparing with free sCT. Results: Tat-sCT mixture gave higher cumulative amounts and fluxes of sCT than free sCT. The maximum percentage of sCT of 58.36 ± 12.33% permeated into the receiving chamber was found in Tat-sCT mixture at 6 h which was 3.50 folds of free sCT. Tat-sCT mixture demonstrated better sCT stability than sCT solution after 1 month storage at 4°C, 25°C and 45°C. Discussion: The positively-charged arginine groups in Tat might be responsible for the binding of peptide complexes to negatively charged cell surfaces by electrostatic interactions and also the translocation of sCT through the excised skin. Conclusion: This study demonstrated the enhancements of transdermal absorption and stability of sCT by Tat peptide with potential for further application in transdermal delivery of other therapeutic peptides. © 2013 Informa Healthcare USA, Inc.
format Journal
author Jiradej Manosroi
Warangkana Lohcharoenkal
Friedrich Götz
Rolf G. Werner
Worapaka Manosroi
Aranya Manosroi
author_facet Jiradej Manosroi
Warangkana Lohcharoenkal
Friedrich Götz
Rolf G. Werner
Worapaka Manosroi
Aranya Manosroi
author_sort Jiradej Manosroi
title Transdermal absorption and stability enhancement of salmon calcitonin by Tat peptide
title_short Transdermal absorption and stability enhancement of salmon calcitonin by Tat peptide
title_full Transdermal absorption and stability enhancement of salmon calcitonin by Tat peptide
title_fullStr Transdermal absorption and stability enhancement of salmon calcitonin by Tat peptide
title_full_unstemmed Transdermal absorption and stability enhancement of salmon calcitonin by Tat peptide
title_sort transdermal absorption and stability enhancement of salmon calcitonin by tat peptide
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84874503901&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52401
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