Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study

Introduction: A 3-antibiotic combination (3Mix) is widely used in endodontics for root canal disinfection, particularly in pulp revascularization procedures. However, the cytotoxicity of 3Mix has not been evaluated. The purpose of this study was to determine the cytotoxicity and antibacterial effica...

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Main Authors: Sorapong Chuensombat, Saengusa Khemaleelakul, Siriporn Chattipakorn, Tanida Srisuwan
Format: Journal
Published: 2018
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spelling th-cmuir.6653943832-524752018-09-04T09:25:47Z Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study Sorapong Chuensombat Saengusa Khemaleelakul Siriporn Chattipakorn Tanida Srisuwan Dentistry Introduction: A 3-antibiotic combination (3Mix) is widely used in endodontics for root canal disinfection, particularly in pulp revascularization procedures. However, the cytotoxicity of 3Mix has not been evaluated. The purpose of this study was to determine the cytotoxicity and antibacterial efficacy of 3Mix and each single antibiotic component of 3Mix. Methods: For the cytotoxicity test, human dental pulp cells and apical papilla cells were exposed to either 3Mix or to each single antibiotic component of 3Mix using concentrations of 0.024, 0.097, 0.39, 1.56, 6.25, and 25.00 μg/mL for 1, 3, 5, and 7 days. Cell viability was determined using the 3-[4,5-dimethylthiazol-2- yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. For the antibacterial test, 25.00 μg/mL and 0.39 μg/mL 3Mix or single antibiotic were tested on bacteria isolated from necrotic teeth by measuring bacterial recovery on blood agar. Results: The 0.024-μg/mL concentration of all experimental groups generated the highest dental pulp cell or apical pulp cell viability at all time periods. On day 7, 0.39 μg/mL 3Mix produced more than 90% cell viability; 25.00 μg/mL 3Mix completely eliminated isolated bacteria, whereas 0.39 μg/mL was unable to eradicate all bacteria. However, the overall bacterial reduction was significantly different compared with the control group (P < .01). Conclusions: All drugs except metronidazole induced cytotoxicity on cultured cells. 3Mix generated higher cytotoxicity compared with a single drug. The cytotoxicity increased in a concentration- and time-dependent manner; 0.39 μg/mL 3Mix had less cytotoxicity and was able to significantly reduce bacteria isolated from necrotic teeth. Copyright © 2013 American Association of Endodontists. 2018-09-04T09:25:47Z 2018-09-04T09:25:47Z 2013-06-01 Journal 00992399 2-s2.0-84877875951 10.1016/j.joen.2012.11.041 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877875951&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52475
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Dentistry
spellingShingle Dentistry
Sorapong Chuensombat
Saengusa Khemaleelakul
Siriporn Chattipakorn
Tanida Srisuwan
Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
description Introduction: A 3-antibiotic combination (3Mix) is widely used in endodontics for root canal disinfection, particularly in pulp revascularization procedures. However, the cytotoxicity of 3Mix has not been evaluated. The purpose of this study was to determine the cytotoxicity and antibacterial efficacy of 3Mix and each single antibiotic component of 3Mix. Methods: For the cytotoxicity test, human dental pulp cells and apical papilla cells were exposed to either 3Mix or to each single antibiotic component of 3Mix using concentrations of 0.024, 0.097, 0.39, 1.56, 6.25, and 25.00 μg/mL for 1, 3, 5, and 7 days. Cell viability was determined using the 3-[4,5-dimethylthiazol-2- yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. For the antibacterial test, 25.00 μg/mL and 0.39 μg/mL 3Mix or single antibiotic were tested on bacteria isolated from necrotic teeth by measuring bacterial recovery on blood agar. Results: The 0.024-μg/mL concentration of all experimental groups generated the highest dental pulp cell or apical pulp cell viability at all time periods. On day 7, 0.39 μg/mL 3Mix produced more than 90% cell viability; 25.00 μg/mL 3Mix completely eliminated isolated bacteria, whereas 0.39 μg/mL was unable to eradicate all bacteria. However, the overall bacterial reduction was significantly different compared with the control group (P < .01). Conclusions: All drugs except metronidazole induced cytotoxicity on cultured cells. 3Mix generated higher cytotoxicity compared with a single drug. The cytotoxicity increased in a concentration- and time-dependent manner; 0.39 μg/mL 3Mix had less cytotoxicity and was able to significantly reduce bacteria isolated from necrotic teeth. Copyright © 2013 American Association of Endodontists.
format Journal
author Sorapong Chuensombat
Saengusa Khemaleelakul
Siriporn Chattipakorn
Tanida Srisuwan
author_facet Sorapong Chuensombat
Saengusa Khemaleelakul
Siriporn Chattipakorn
Tanida Srisuwan
author_sort Sorapong Chuensombat
title Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
title_short Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
title_full Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
title_fullStr Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
title_full_unstemmed Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
title_sort cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: an in vitro study
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877875951&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52475
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