Early detection of trichinella spiralis in muscle of infected mice by real-time fluorescence resonance energy transfer PCR

• Main Authors: Chairat Tantrawatpan, Pewpan M. Intapan, Tongjit Thanchomnang, Oranuch Sanpool, Penchom Janwan, Thidarut Boonmars, Nimit Morakote, Wanchai Maleewong
• Format: Journal
• Published: 2018
• Subjects: Immunology and Microbiology; Medicine
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84884223269&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/52628
• Institution: Chiang Mai University

Real-time fluorescence resonance energy transfer (FRET) PCR and melting curve analysis using newly developed fluorophore-labeled hybridization probes were applied for the detection of Trichinella spiralis DNA in muscle of mice following oral inoculation with 300 T. spiralis larvae. The developed ass...