Plasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virus

Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses the pro-inflammatory immune response following infection of myeloid antigen-presenting cells. A reduced pro-inflammatory immune response modulates PRRSV replication, clinical disease, and persistent infection of the virus. Numero...

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Main Authors: Wasin Charerntantanakul, Surangkanang Yamkanchoo, Watchara Kasinrerk
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/52648
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spelling th-cmuir.6653943832-526482018-09-04T09:38:13Z Plasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virus Wasin Charerntantanakul Surangkanang Yamkanchoo Watchara Kasinrerk Immunology and Microbiology Veterinary Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses the pro-inflammatory immune response following infection of myeloid antigen-presenting cells. A reduced pro-inflammatory immune response modulates PRRSV replication, clinical disease, and persistent infection of the virus. Numerous efforts have been made to enhance the pro-inflammatory immune response to PRRSV, but only a few attempts have so far elicited satisfactory results. The present study aims to evaluate in vitro the potential of plasmids expressing porcine interferon gamma (pcDNA-IFNγ) to enhance the expression of pro-inflammatory immune parameters in PRRSV-inoculated monocytes. Naïve blood monocytes from eight PRRSV-seronegative pigs were inoculated with PRRSV and subsequently transfected with pcDNA-IFNγ or pcDNA (empty plasmid vector) and stimulated with lipopolysaccharide (LPS). The mRNA expression levels of IFNγ, interleukin-1 beta (IL-1β), IL-10, IL-12p40, tumor necrosis factor alpha (TNFα), transforming growth factor beta (TGFβ), CD80, and CD86 were evaluated by real-time PCR. The IFNγ, IL-10, and TNFα protein production was determined by ELISA. Compared with PRRSV-inoculated monocyte control, transfection with pcDNA-IFNγ, but not pcDNA, significantly enhanced IFNγ, TNFα, CD80, and CD86 mRNA expression, and IFNγ and TNFα protein production. A slight increase in IL-1β and IL-12p40 mRNA expression was also observed. Neither pcDNA-IFNγ nor pcDNA transfection affected IL-10 and TGFβ expression. Our results thus suggest that pcDNA-IFNγ may be an effective immunostimulator for potentiating the pro-inflammatory immune response to PRRSV. © 2013 Elsevier B.V. 2018-09-04T09:28:55Z 2018-09-04T09:28:55Z 2013-03-18 Journal 18732534 01652427 2-s2.0-84884728296 10.1016/j.vetimm.2013.02.013 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84884728296&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52648
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Veterinary
spellingShingle Immunology and Microbiology
Veterinary
Wasin Charerntantanakul
Surangkanang Yamkanchoo
Watchara Kasinrerk
Plasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virus
description Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses the pro-inflammatory immune response following infection of myeloid antigen-presenting cells. A reduced pro-inflammatory immune response modulates PRRSV replication, clinical disease, and persistent infection of the virus. Numerous efforts have been made to enhance the pro-inflammatory immune response to PRRSV, but only a few attempts have so far elicited satisfactory results. The present study aims to evaluate in vitro the potential of plasmids expressing porcine interferon gamma (pcDNA-IFNγ) to enhance the expression of pro-inflammatory immune parameters in PRRSV-inoculated monocytes. Naïve blood monocytes from eight PRRSV-seronegative pigs were inoculated with PRRSV and subsequently transfected with pcDNA-IFNγ or pcDNA (empty plasmid vector) and stimulated with lipopolysaccharide (LPS). The mRNA expression levels of IFNγ, interleukin-1 beta (IL-1β), IL-10, IL-12p40, tumor necrosis factor alpha (TNFα), transforming growth factor beta (TGFβ), CD80, and CD86 were evaluated by real-time PCR. The IFNγ, IL-10, and TNFα protein production was determined by ELISA. Compared with PRRSV-inoculated monocyte control, transfection with pcDNA-IFNγ, but not pcDNA, significantly enhanced IFNγ, TNFα, CD80, and CD86 mRNA expression, and IFNγ and TNFα protein production. A slight increase in IL-1β and IL-12p40 mRNA expression was also observed. Neither pcDNA-IFNγ nor pcDNA transfection affected IL-10 and TGFβ expression. Our results thus suggest that pcDNA-IFNγ may be an effective immunostimulator for potentiating the pro-inflammatory immune response to PRRSV. © 2013 Elsevier B.V.
format Journal
author Wasin Charerntantanakul
Surangkanang Yamkanchoo
Watchara Kasinrerk
author_facet Wasin Charerntantanakul
Surangkanang Yamkanchoo
Watchara Kasinrerk
author_sort Wasin Charerntantanakul
title Plasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virus
title_short Plasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virus
title_full Plasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virus
title_fullStr Plasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virus
title_full_unstemmed Plasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virus
title_sort plasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virus
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84884728296&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52648
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