HPV genotyping in adenocarcinoma of the uterine cervix in Thailand

Objective To determine the distribution of human papillomavirus (HPV) genotypes in cervical adenocarcinoma in Thailand and to evaluate the clinicopathologic characteristics associated with common HPV genotypes. Methods Formalin-fixed, paraffin-embedded tissues from 150 patients with adenocarcinoma w...

Full description

Saved in:
Bibliographic Details
Main Authors: Sumalee Siriaunkgul, Utaiwan Utaipat, Cheepsumon Suthipintawong, Kobkul Tungsinmunkong, Surang Triratanachat, Surapan Khunamornpong
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84887101288&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52940
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-52940
record_format dspace
spelling th-cmuir.6653943832-529402018-09-04T09:34:56Z HPV genotyping in adenocarcinoma of the uterine cervix in Thailand Sumalee Siriaunkgul Utaiwan Utaipat Cheepsumon Suthipintawong Kobkul Tungsinmunkong Surang Triratanachat Surapan Khunamornpong Medicine Objective To determine the distribution of human papillomavirus (HPV) genotypes in cervical adenocarcinoma in Thailand and to evaluate the clinicopathologic characteristics associated with common HPV genotypes. Methods Formalin-fixed, paraffin-embedded tissues from 150 patients with adenocarcinoma were collected from 4 areas of Thailand. Infection with HPV was detected by nested polymerase chain reaction (PCR) with primers MY09/11 and GP5 +/6 +. Genotyping was performed using a linear array assay, followed by type-specific PCR targeting the E6/E7 regions of HPV-16, HPV-18, and HPV-52 if the linear array test was negative. Results Human papillomavirus DNA was detected in 145 (97%) adenocarcinomas (132 single infections; 11 multiple infections; 2 tumors with undetermined HPV type). Genotype 18 was most common (66%), followed by HPV-16 (30%) and HPV-45 (3%). Infection with only HPV-16 and/or HPV-18 accounted for 88% of the HPV-positive tumors. Patients with HPV-18 infection had a younger age (P = 0.009) and higher tumor grade (P < 0.001) than patients with HPV-16 infection. Conclusion The HPV detection rate in cervical adenocarcinomas in Thailand is high. The predominant genotype is HPV-18, being twice as common as HPV-16. Genotype variations are associated with patient age and tumor grade. Vaccination against HPV-16/HPV-18 might prevent almost 90% of adenocarcinomas. © 2013 International Federation of Gynecology and Obstetrics. 2018-09-04T09:34:56Z 2018-09-04T09:34:56Z 2013-01-01 Journal 18793479 00207292 2-s2.0-84887101288 10.1016/j.ijgo.2013.06.034 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84887101288&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52940
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Sumalee Siriaunkgul
Utaiwan Utaipat
Cheepsumon Suthipintawong
Kobkul Tungsinmunkong
Surang Triratanachat
Surapan Khunamornpong
HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
description Objective To determine the distribution of human papillomavirus (HPV) genotypes in cervical adenocarcinoma in Thailand and to evaluate the clinicopathologic characteristics associated with common HPV genotypes. Methods Formalin-fixed, paraffin-embedded tissues from 150 patients with adenocarcinoma were collected from 4 areas of Thailand. Infection with HPV was detected by nested polymerase chain reaction (PCR) with primers MY09/11 and GP5 +/6 +. Genotyping was performed using a linear array assay, followed by type-specific PCR targeting the E6/E7 regions of HPV-16, HPV-18, and HPV-52 if the linear array test was negative. Results Human papillomavirus DNA was detected in 145 (97%) adenocarcinomas (132 single infections; 11 multiple infections; 2 tumors with undetermined HPV type). Genotype 18 was most common (66%), followed by HPV-16 (30%) and HPV-45 (3%). Infection with only HPV-16 and/or HPV-18 accounted for 88% of the HPV-positive tumors. Patients with HPV-18 infection had a younger age (P = 0.009) and higher tumor grade (P < 0.001) than patients with HPV-16 infection. Conclusion The HPV detection rate in cervical adenocarcinomas in Thailand is high. The predominant genotype is HPV-18, being twice as common as HPV-16. Genotype variations are associated with patient age and tumor grade. Vaccination against HPV-16/HPV-18 might prevent almost 90% of adenocarcinomas. © 2013 International Federation of Gynecology and Obstetrics.
format Journal
author Sumalee Siriaunkgul
Utaiwan Utaipat
Cheepsumon Suthipintawong
Kobkul Tungsinmunkong
Surang Triratanachat
Surapan Khunamornpong
author_facet Sumalee Siriaunkgul
Utaiwan Utaipat
Cheepsumon Suthipintawong
Kobkul Tungsinmunkong
Surang Triratanachat
Surapan Khunamornpong
author_sort Sumalee Siriaunkgul
title HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
title_short HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
title_full HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
title_fullStr HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
title_full_unstemmed HPV genotyping in adenocarcinoma of the uterine cervix in Thailand
title_sort hpv genotyping in adenocarcinoma of the uterine cervix in thailand
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84887101288&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52940
_version_ 1681424042963238912