Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei

Penicillium marneffei is a significant opportunistic fungal pathogen in Southeast Asia and its ability to survive inside the host macrophages is believed to be important in the establishment of infection. Previously, we isolated a gene encoding a catalase- peroxidase (cpeA) from P. marneffei and sho...

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Main Authors: Monsicha Pongpom, Pritsana Sawatdeechaikul, Aksarakorn Kummasook, Sophit Khanthawong, Nongnuch Vanittanakom
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/52945
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-529452018-09-04T09:38:19Z Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei Monsicha Pongpom Pritsana Sawatdeechaikul Aksarakorn Kummasook Sophit Khanthawong Nongnuch Vanittanakom Medicine Veterinary Penicillium marneffei is a significant opportunistic fungal pathogen in Southeast Asia and its ability to survive inside the host macrophages is believed to be important in the establishment of infection. Previously, we isolated a gene encoding a catalase- peroxidase (cpeA) from P. marneffei and showed that the cpeA transcript is specifically upregulated during yeast phase growth at 37°C. In this study, the cpeA transcript was found to be induced during the mycelium to yeast phase transition and during stress conditions induced by hydrogen peroxide treatment. Null mutation of cpeA reduced the fungal tolerance to hydrogen peroxide but not to heat stress. These results indicated that the CpeA plays a crucial role in this fungus' oxidative stress response. Western blot analysis demonstrated that the CpeA induced antibody production in P. marneffei-infected patients, including highly exposed-healthy people. This is the first report that the catalase-peroxidase possesses an immunogenic property in fungi. © 2013 ISHAM. 2018-09-04T09:34:58Z 2018-09-04T09:34:58Z 2013-01-01 Journal 14602709 13693786 2-s2.0-84885907409 10.3109/13693786.2013.807445 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84885907409&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52945
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
Veterinary
spellingShingle Medicine
Veterinary
Monsicha Pongpom
Pritsana Sawatdeechaikul
Aksarakorn Kummasook
Sophit Khanthawong
Nongnuch Vanittanakom
Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
description Penicillium marneffei is a significant opportunistic fungal pathogen in Southeast Asia and its ability to survive inside the host macrophages is believed to be important in the establishment of infection. Previously, we isolated a gene encoding a catalase- peroxidase (cpeA) from P. marneffei and showed that the cpeA transcript is specifically upregulated during yeast phase growth at 37°C. In this study, the cpeA transcript was found to be induced during the mycelium to yeast phase transition and during stress conditions induced by hydrogen peroxide treatment. Null mutation of cpeA reduced the fungal tolerance to hydrogen peroxide but not to heat stress. These results indicated that the CpeA plays a crucial role in this fungus' oxidative stress response. Western blot analysis demonstrated that the CpeA induced antibody production in P. marneffei-infected patients, including highly exposed-healthy people. This is the first report that the catalase-peroxidase possesses an immunogenic property in fungi. © 2013 ISHAM.
format Journal
author Monsicha Pongpom
Pritsana Sawatdeechaikul
Aksarakorn Kummasook
Sophit Khanthawong
Nongnuch Vanittanakom
author_facet Monsicha Pongpom
Pritsana Sawatdeechaikul
Aksarakorn Kummasook
Sophit Khanthawong
Nongnuch Vanittanakom
author_sort Monsicha Pongpom
title Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
title_short Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
title_full Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
title_fullStr Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
title_full_unstemmed Antioxidative and immunogenic properties of catalase-peroxidase protein in Penicillium marneffei
title_sort antioxidative and immunogenic properties of catalase-peroxidase protein in penicillium marneffei
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84885907409&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52945
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