Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract
The flowers of African marigold (Tagetes erecta L), a medicinal plant widely cultivated in Thailand, were subjected to evaluation for total phenolics, DPPH scavenging and thiobarbituric acid-reactive substance (TBARs) assays as well as tyrosinase inhibitory activity. In preliminary studies, the ethy...
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th-cmuir.6653943832-529612018-09-04T09:35:35Z Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract Ampai Phrutivorapongkul Kanokwan Kiattisin Pensak Jantrawut Sunee Chansakaow Suwanna Vejabhikul Pimporn Leelapornpisid Pharmacology, Toxicology and Pharmaceutics The flowers of African marigold (Tagetes erecta L), a medicinal plant widely cultivated in Thailand, were subjected to evaluation for total phenolics, DPPH scavenging and thiobarbituric acid-reactive substance (TBARs) assays as well as tyrosinase inhibitory activity. In preliminary studies, the ethyl acetate (EA) extract obtained by continuous extraction showed the highest activities with highest phenolic content among all extracts. Bioassay-guided fractionation of EA extract led to isolation of a flavonoid identified as quercetagetin. Interestingly, it was found that quercetagetin exhibited potent DPPH scavenging activity with IC50 of 3.70 μg/ml which is about 2-3 times higher activity than standard quercetin (IC50 5.07 μg/ml) and trolox (IC50 9.93 μg/ml). Moreover, it exhibited tyrosinase inhibitory activity on L-tyrosine (IC50 89.31 μg/ml), higher than α- and β-arbutins (IC50 157.77 and 222.35 μg/ml) and slightly higher (IC50 128.41 μg/ml) than ellagic acid (IC 50 151.1 μg/ml) when using L-DOPA as substrate. Testing with skin fibroblasts, all the extracts and quercetagetin demonstrated no toxic effect. These finding strongly indicate that African marigold flower is a promising source of natural antioxidative and tyrosinase inhibitory substances with safe to skin. 2018-09-04T09:35:35Z 2018-09-04T09:35:35Z 2013-11-01 Journal 1011601X 2-s2.0-84892577413 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84892577413&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52961 |
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Pharmacology, Toxicology and Pharmaceutics Ampai Phrutivorapongkul Kanokwan Kiattisin Pensak Jantrawut Sunee Chansakaow Suwanna Vejabhikul Pimporn Leelapornpisid Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract |
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The flowers of African marigold (Tagetes erecta L), a medicinal plant widely cultivated in Thailand, were subjected to evaluation for total phenolics, DPPH scavenging and thiobarbituric acid-reactive substance (TBARs) assays as well as tyrosinase inhibitory activity. In preliminary studies, the ethyl acetate (EA) extract obtained by continuous extraction showed the highest activities with highest phenolic content among all extracts. Bioassay-guided fractionation of EA extract led to isolation of a flavonoid identified as quercetagetin. Interestingly, it was found that quercetagetin exhibited potent DPPH scavenging activity with IC50 of 3.70 μg/ml which is about 2-3 times higher activity than standard quercetin (IC50 5.07 μg/ml) and trolox (IC50 9.93 μg/ml). Moreover, it exhibited tyrosinase inhibitory activity on L-tyrosine (IC50 89.31 μg/ml), higher than α- and β-arbutins (IC50 157.77 and 222.35 μg/ml) and slightly higher (IC50 128.41 μg/ml) than ellagic acid (IC 50 151.1 μg/ml) when using L-DOPA as substrate. Testing with skin fibroblasts, all the extracts and quercetagetin demonstrated no toxic effect. These finding strongly indicate that African marigold flower is a promising source of natural antioxidative and tyrosinase inhibitory substances with safe to skin. |
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Ampai Phrutivorapongkul Kanokwan Kiattisin Pensak Jantrawut Sunee Chansakaow Suwanna Vejabhikul Pimporn Leelapornpisid |
author_facet |
Ampai Phrutivorapongkul Kanokwan Kiattisin Pensak Jantrawut Sunee Chansakaow Suwanna Vejabhikul Pimporn Leelapornpisid |
author_sort |
Ampai Phrutivorapongkul |
title |
Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract |
title_short |
Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract |
title_full |
Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract |
title_fullStr |
Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract |
title_full_unstemmed |
Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract |
title_sort |
appraisal of biological activities and identification of phenolic compound of african marigold (tagetes erecta) flower extract |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84892577413&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52961 |
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