Genomic DNA isolation from dried blood using gelatin-coated magnetic particles

Genomic DNA isolation from dried blood using gelatin-coated magnetic particles

Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability deter...

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Main Authors: Sorasak Intorasoot, Jintara Techateerawat, Amornrat Intorasoot
Format: Journal
Published: 2018
Subjects:
Multidisciplinary
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880650274&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53035
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-530352018-09-04T09:38:33Z Genomic DNA isolation from dried blood using gelatin-coated magnetic particles Sorasak Intorasoot Jintara Techateerawat Amornrat Intorasoot Multidisciplinary Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability determination. Approximately three times higher DNA yield was recovered by coated magnetic particles than the silicabased kit. In addition, the quality of extracted DNA was determined using β-actin gene amplification by polymerase chain reaction (PCR). It was implied that genomic DNA was stable for at least 28 days in a dried condition. Gelatin-coated magnetic particles are rapid, simple and useful for DNA extraction in field applications. 2018-09-04T09:38:33Z 2018-09-04T09:38:33Z 2013-07-01 Journal 00113891 2-s2.0-84880650274 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880650274&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53035
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Multidisciplinary
spellingShingle Multidisciplinary
Sorasak Intorasoot
Jintara Techateerawat
Amornrat Intorasoot
Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
description Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability determination. Approximately three times higher DNA yield was recovered by coated magnetic particles than the silicabased kit. In addition, the quality of extracted DNA was determined using β-actin gene amplification by polymerase chain reaction (PCR). It was implied that genomic DNA was stable for at least 28 days in a dried condition. Gelatin-coated magnetic particles are rapid, simple and useful for DNA extraction in field applications.
format Journal
author Sorasak Intorasoot
Jintara Techateerawat
Amornrat Intorasoot
author_facet Sorasak Intorasoot
Jintara Techateerawat
Amornrat Intorasoot
author_sort Sorasak Intorasoot
title Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_short Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_full Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_fullStr Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_full_unstemmed Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_sort genomic dna isolation from dried blood using gelatin-coated magnetic particles
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880650274&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53035
_version_ 1681424060443000832

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