Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus
In this study, we focused on the effect of glutamine synthetase (GSI) activity in Mesorhizobium loti on the symbiosis between the host plant, Lotus japonicus, and the bacteroids. We used a signature-tagged mutant of M. loti (STM30) with a transposon inserted into the GSI (mll0343) gene. The L. japon...
Saved in:
Main Authors: | , , , , , , , |
---|---|
Format: | Journal |
Published: |
2018
|
Subjects: | |
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893203432&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53083 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
id |
th-cmuir.6653943832-53083 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-530832018-09-04T09:45:01Z Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus Sirinapa Chungopast Pilunthana Thapanapongworakul Hiroyuki Matsuura Tan Van Dao Toshimasa Asahi Kuninao Tada Shigeyuki Tajima Mika Nomura Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology In this study, we focused on the effect of glutamine synthetase (GSI) activity in Mesorhizobium loti on the symbiosis between the host plant, Lotus japonicus, and the bacteroids. We used a signature-tagged mutant of M. loti (STM30) with a transposon inserted into the GSI (mll0343) gene. The L. japonicus plants inoculated with STM30 had significantly more nodules, and the occurrence of senesced nodules was much higher than in plants inoculated with the wild-type. The acetylene reduction activity (ARA) per nodule inoculated with STM30 was lowered compared to the control. Also, the concentration of chlorophyll, glutamine, and asparagine in leaves of STM30-infected plants was found to be reduced. Taken together, these data demonstrate that a GSI deficiency in M. loti differentially affects legume-rhizobia symbiosis by modifying nodule development and metabolic processes. © 2013 Elsevier GmbH. 2018-09-04T09:43:32Z 2018-09-04T09:43:32Z 2014-03-01 Journal 01761617 2-s2.0-84893203432 10.1016/j.jplph.2013.10.015 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893203432&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53083 |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
topic |
Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology |
spellingShingle |
Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Sirinapa Chungopast Pilunthana Thapanapongworakul Hiroyuki Matsuura Tan Van Dao Toshimasa Asahi Kuninao Tada Shigeyuki Tajima Mika Nomura Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus |
description |
In this study, we focused on the effect of glutamine synthetase (GSI) activity in Mesorhizobium loti on the symbiosis between the host plant, Lotus japonicus, and the bacteroids. We used a signature-tagged mutant of M. loti (STM30) with a transposon inserted into the GSI (mll0343) gene. The L. japonicus plants inoculated with STM30 had significantly more nodules, and the occurrence of senesced nodules was much higher than in plants inoculated with the wild-type. The acetylene reduction activity (ARA) per nodule inoculated with STM30 was lowered compared to the control. Also, the concentration of chlorophyll, glutamine, and asparagine in leaves of STM30-infected plants was found to be reduced. Taken together, these data demonstrate that a GSI deficiency in M. loti differentially affects legume-rhizobia symbiosis by modifying nodule development and metabolic processes. © 2013 Elsevier GmbH. |
format |
Journal |
author |
Sirinapa Chungopast Pilunthana Thapanapongworakul Hiroyuki Matsuura Tan Van Dao Toshimasa Asahi Kuninao Tada Shigeyuki Tajima Mika Nomura |
author_facet |
Sirinapa Chungopast Pilunthana Thapanapongworakul Hiroyuki Matsuura Tan Van Dao Toshimasa Asahi Kuninao Tada Shigeyuki Tajima Mika Nomura |
author_sort |
Sirinapa Chungopast |
title |
Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus |
title_short |
Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus |
title_full |
Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus |
title_fullStr |
Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus |
title_full_unstemmed |
Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus |
title_sort |
glutamine synthetase i-deficiency in mesorhizobium loti differentially affects nodule development and activity in lotus japonicus |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893203432&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53083 |
_version_ |
1681424069342265344 |