A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion

Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies...

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Main Authors:	Supansa Pata, Saichit Khummuang, Sakorn Pornprasert, Thanusak Tatu, Watchara Kasinrerk
Format:	Journal
Published:	2018
Subjects:	Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine
Online Access:	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84890324046&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53187
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Institution:	Chiang Mai University

id	th-cmuir.6653943832-53187
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spelling	th-cmuir.6653943832-531872018-09-04T09:56:59Z A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion Supansa Pata Saichit Khummuang Sakorn Pornprasert Thanusak Tatu Watchara Kasinrerk Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies specific to the ζ-globin chain without cross reaction with other globin chains. A simple and sensitive ELISA was developed by using poly-l-lysine to increase the protein binding to the ELISA plate. The developed poly-l-lysine pre-coated ELISA has a very high sensitivity (100%) and specificity (97%) for detection of carriers of α-thalassemia-1 with Southeast Asian-type deletion. © 2014 Copyright Taylor and Francis Group, LLC. 2018-09-04T09:44:58Z 2018-09-04T09:44:58Z 2014-04-03 Journal 15324230 15321819 2-s2.0-84890324046 10.1080/15321819.2013.838963 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84890324046&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53187
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
topic	Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine
spellingShingle	Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine Supansa Pata Saichit Khummuang Sakorn Pornprasert Thanusak Tatu Watchara Kasinrerk A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
description	Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies specific to the ζ-globin chain without cross reaction with other globin chains. A simple and sensitive ELISA was developed by using poly-l-lysine to increase the protein binding to the ELISA plate. The developed poly-l-lysine pre-coated ELISA has a very high sensitivity (100%) and specificity (97%) for detection of carriers of α-thalassemia-1 with Southeast Asian-type deletion. © 2014 Copyright Taylor and Francis Group, LLC.
format	Journal
author	Supansa Pata Saichit Khummuang Sakorn Pornprasert Thanusak Tatu Watchara Kasinrerk
author_facet	Supansa Pata Saichit Khummuang Sakorn Pornprasert Thanusak Tatu Watchara Kasinrerk
author_sort	Supansa Pata
title	A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
title_short	A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
title_full	A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
title_fullStr	A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
title_full_unstemmed	A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
title_sort	simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
publishDate	2018
url	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84890324046&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53187
_version_	1681424088663326720

A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion

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