Using green fluorescent protein fusion approach to demonstrate the putative laccase-encoding gene expression and an importance of 3′ untranslated region in gene expression in Penicillium marneffei
Penicillium marneffei is an opportunistic fungus that can cause a fatal disseminated infection in immunocompromised patients. Understanding the biology and pathogenic capacity of this fungus is essential for medical treatment and infection control. Genetic transformation has been developed to invest...
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th-cmuir.6653943832-532312018-09-04T10:01:35Z Using green fluorescent protein fusion approach to demonstrate the putative laccase-encoding gene expression and an importance of 3′ untranslated region in gene expression in Penicillium marneffei Ariya Sapmak Sirida Youngchim Nongnuch Vanittanakom Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy Penicillium marneffei is an opportunistic fungus that can cause a fatal disseminated infection in immunocompromised patients. Understanding the biology and pathogenic capacity of this fungus is essential for medical treatment and infection control. Genetic transformation has been developed to investigate the expression and function of the gene of interest in pathogenic fungi including P. marneffei. To date, the whole genome sequence of P. marneffei has been submitted to an open access GenBank database enhancing the capability of genetic analysis. The genetic analysis provides an insight in its biology and pathogenicity and an option of a rational target for drug development. In this study, we fused the enhanced green fluorescent protein (egfp) gene into a putative laccase-encoding gene (arb2) of P. marneffei to demonstrate gene expression and the effect of 3′ untranslated region (UTR) to the production of protein. Our findings highlight the necessary of keeping a native promoter and 3′ UTR for gene expression analysis. Many considerations are described and demonstrated for successful fusion protein production. These data should be beneficial for further studies including gene expression, protein localization, and the role of gene and nucleotide sequences. 2018-09-04T09:45:35Z 2018-09-04T09:45:35Z 2014-01-01 Journal 01252526 2-s2.0-84899630834 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899630834&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53231 |
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Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy Ariya Sapmak Sirida Youngchim Nongnuch Vanittanakom Using green fluorescent protein fusion approach to demonstrate the putative laccase-encoding gene expression and an importance of 3′ untranslated region in gene expression in Penicillium marneffei |
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Penicillium marneffei is an opportunistic fungus that can cause a fatal disseminated infection in immunocompromised patients. Understanding the biology and pathogenic capacity of this fungus is essential for medical treatment and infection control. Genetic transformation has been developed to investigate the expression and function of the gene of interest in pathogenic fungi including P. marneffei. To date, the whole genome sequence of P. marneffei has been submitted to an open access GenBank database enhancing the capability of genetic analysis. The genetic analysis provides an insight in its biology and pathogenicity and an option of a rational target for drug development. In this study, we fused the enhanced green fluorescent protein (egfp) gene into a putative laccase-encoding gene (arb2) of P. marneffei to demonstrate gene expression and the effect of 3′ untranslated region (UTR) to the production of protein. Our findings highlight the necessary of keeping a native promoter and 3′ UTR for gene expression analysis. Many considerations are described and demonstrated for successful fusion protein production. These data should be beneficial for further studies including gene expression, protein localization, and the role of gene and nucleotide sequences. |
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Ariya Sapmak Sirida Youngchim Nongnuch Vanittanakom |
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Ariya Sapmak Sirida Youngchim Nongnuch Vanittanakom |
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Ariya Sapmak |
title |
Using green fluorescent protein fusion approach to demonstrate the putative laccase-encoding gene expression and an importance of 3′ untranslated region in gene expression in Penicillium marneffei |
title_short |
Using green fluorescent protein fusion approach to demonstrate the putative laccase-encoding gene expression and an importance of 3′ untranslated region in gene expression in Penicillium marneffei |
title_full |
Using green fluorescent protein fusion approach to demonstrate the putative laccase-encoding gene expression and an importance of 3′ untranslated region in gene expression in Penicillium marneffei |
title_fullStr |
Using green fluorescent protein fusion approach to demonstrate the putative laccase-encoding gene expression and an importance of 3′ untranslated region in gene expression in Penicillium marneffei |
title_full_unstemmed |
Using green fluorescent protein fusion approach to demonstrate the putative laccase-encoding gene expression and an importance of 3′ untranslated region in gene expression in Penicillium marneffei |
title_sort |
using green fluorescent protein fusion approach to demonstrate the putative laccase-encoding gene expression and an importance of 3′ untranslated region in gene expression in penicillium marneffei |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899630834&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53231 |
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