Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production

© 2014 Kanchana Dumri and Dau Hung Anh. Biodiesel production technology is competitive in terms of low cost and alternative source of energy which should be not only sustainable but also environmentally friendly. Designing of the lipase immobilization for biodiesel production has a remarkable impact...

Full description

Saved in:
Bibliographic Details
Main Authors: Kanchana Dumri, Dau Hung Anh
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84934922665&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53247
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-53247
record_format dspace
spelling th-cmuir.6653943832-532472018-09-04T09:45:47Z Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production Kanchana Dumri Dau Hung Anh Biochemistry, Genetics and Molecular Biology © 2014 Kanchana Dumri and Dau Hung Anh. Biodiesel production technology is competitive in terms of low cost and alternative source of energy which should be not only sustainable but also environmentally friendly. Designing of the lipase immobilization for biodiesel production has a remarkable impact and is still challenging. In this work, biodiesel production from soybean oil was enhanced and facilitated by using a novel biocatalyst consisting of commercial lipase (EC 3.1.1.3), silver nanoparticles, and polydopamine. Silver nanoparticles (AgNPs) were synthesized with a size range of 10-20 nm. Polydopamine (PD) was delivered by the self-polymerization of dopamine in 10 mM Tris-HCl pH 8.5 and simultaneously coated the AgNPs to form a PD/AgNPs complex. Lipase was immobilized on the PD/AgNPs complex surface via covalent bonds to form a tailor-made biocatalyst consisting of immobilized lipase/PD/AgNPs complex (LPA). The formation and morphology of each composition were characterized by UV-Vis spectroscopy and scanning electron microscope (SEM). Significantly, gas chromatography analysis showed a remarkable biodiesel production yield of 95% by using the LPA complex at 40°C for 6-hours reaction time, whereas the yield was 86% when using free lyophilized lipase. The LPA complex was apparently reusable after 7 batches and the latter conversion rate of soybean oil was decreased by only 27%. 2018-09-04T09:45:47Z 2018-09-04T09:45:47Z 2014-01-01 Journal 20900414 20900406 2-s2.0-84934922665 10.1155/2014/389739 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84934922665&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53247
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Kanchana Dumri
Dau Hung Anh
Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production
description © 2014 Kanchana Dumri and Dau Hung Anh. Biodiesel production technology is competitive in terms of low cost and alternative source of energy which should be not only sustainable but also environmentally friendly. Designing of the lipase immobilization for biodiesel production has a remarkable impact and is still challenging. In this work, biodiesel production from soybean oil was enhanced and facilitated by using a novel biocatalyst consisting of commercial lipase (EC 3.1.1.3), silver nanoparticles, and polydopamine. Silver nanoparticles (AgNPs) were synthesized with a size range of 10-20 nm. Polydopamine (PD) was delivered by the self-polymerization of dopamine in 10 mM Tris-HCl pH 8.5 and simultaneously coated the AgNPs to form a PD/AgNPs complex. Lipase was immobilized on the PD/AgNPs complex surface via covalent bonds to form a tailor-made biocatalyst consisting of immobilized lipase/PD/AgNPs complex (LPA). The formation and morphology of each composition were characterized by UV-Vis spectroscopy and scanning electron microscope (SEM). Significantly, gas chromatography analysis showed a remarkable biodiesel production yield of 95% by using the LPA complex at 40°C for 6-hours reaction time, whereas the yield was 86% when using free lyophilized lipase. The LPA complex was apparently reusable after 7 batches and the latter conversion rate of soybean oil was decreased by only 27%.
format Journal
author Kanchana Dumri
Dau Hung Anh
author_facet Kanchana Dumri
Dau Hung Anh
author_sort Kanchana Dumri
title Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production
title_short Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production
title_full Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production
title_fullStr Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production
title_full_unstemmed Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production
title_sort immobilization of lipase on silver nanoparticles via adhesive polydopamine for biodiesel production
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84934922665&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53247
_version_ 1681424099651354624