High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand

• Main Authors: Sripalwit P., Wongsawad C., Wongsawad P., Anuntalabhochai S.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-33751186329&partnerID=40&md5=c765358d47e2d16e7f4093dc2b32c165; http://www.ncbi.nlm.nih.gov/pubmed/16889772; http://cmuir.cmu.ac.th/handle/6653943832/5326
• Institution: Chiang Mai University

The rumen flukes of 37 cows (Bos indicus) from Chiang Mai and Lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). Three species were found: Paramphistomum epiclitum, Orthocoelium streptocoelium, and Fischoederius elongatus with prevalences of infection of 75.68%, 48.65%, and 40.54%, respectively. Genomic DNA was amplified by polymerase chain reaction based on the high annealing temperature-random amplification of polymorphic DNA (HAT-RAPD) technique. Five random 10-mer oligonucleotide primers (OPA2, OPA4, OPB18, OPC9, and OPH11) produced distinct banding patterns in three species. No genetic variations in these three species were identified using 10 arbitary primers. © 2006 Elsevier Inc. All rights reserved.