Morphological and protein analyses of adult female salivary glands of Anopheles barbirostris species A1 (Diptera: Culicidae)

Morphology and protein profiles of female salivary glands of Anopheles barbirostris species A1 were analyzed. Female glands consisted of a distinctive tri-lobed structure connected to a main salivary canal, a single medial and two lateral lobes with proximal and distal portions. Cellular architectur...

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Main Authors: B. Phattanawiboon, N. Jariyapan, S. Roytrakul, A. Paemanee, S. Sor-suwan, N. Intakhan, W. Chanmol, P. Siriyasatien, A. Saeung, W. Choochote
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/53708
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-537082018-09-04T09:56:14Z Morphological and protein analyses of adult female salivary glands of Anopheles barbirostris species A1 (Diptera: Culicidae) B. Phattanawiboon N. Jariyapan S. Roytrakul A. Paemanee S. Sor-suwan N. Intakhan W. Chanmol P. Siriyasatien A. Saeung W. Choochote Medicine Morphology and protein profiles of female salivary glands of Anopheles barbirostris species A1 were analyzed. Female glands consisted of a distinctive tri-lobed structure connected to a main salivary canal, a single medial and two lateral lobes with proximal and distal portions. Cellular architecture was similar among the lobes, with secretory material appearing as large masses. Cells of the proximal-lateral lobes contained secretory masses with a finely filamentous aspect. In the distal-lateral lobes, cells had a dense secretory product with mottled pattern. Cells of the medial lobe had secretory masses which were uniformly stained and highly electron dense. Following emergence, the glands accumulated secretory material rapidly and developed completely within three days. Degenerative changes including loss of stored secretion and increase of cytoplasmic vacuolation and concentric lamellar structures were observed from day 16 post emergence that correlated with total amount of the salivary gland proteins determined during development. SDS-PAGE, nanoLC-MS, and glycoprotein analysis revealed at least eleven major protein bands, of which each morphological region contained different major proteins. Two glycoproteins, apyrase/5'-nucleotidase and D7, were identified. These results form a basis for further studies on details of cytopathological changes of malarial infected glands and roles of the proteins in disease transmission. 2018-09-04T09:56:14Z 2018-09-04T09:56:14Z 2014-12-01 Journal 01275720 2-s2.0-84936943361 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84936943361&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53708
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
B. Phattanawiboon
N. Jariyapan
S. Roytrakul
A. Paemanee
S. Sor-suwan
N. Intakhan
W. Chanmol
P. Siriyasatien
A. Saeung
W. Choochote
Morphological and protein analyses of adult female salivary glands of Anopheles barbirostris species A1 (Diptera: Culicidae)
description Morphology and protein profiles of female salivary glands of Anopheles barbirostris species A1 were analyzed. Female glands consisted of a distinctive tri-lobed structure connected to a main salivary canal, a single medial and two lateral lobes with proximal and distal portions. Cellular architecture was similar among the lobes, with secretory material appearing as large masses. Cells of the proximal-lateral lobes contained secretory masses with a finely filamentous aspect. In the distal-lateral lobes, cells had a dense secretory product with mottled pattern. Cells of the medial lobe had secretory masses which were uniformly stained and highly electron dense. Following emergence, the glands accumulated secretory material rapidly and developed completely within three days. Degenerative changes including loss of stored secretion and increase of cytoplasmic vacuolation and concentric lamellar structures were observed from day 16 post emergence that correlated with total amount of the salivary gland proteins determined during development. SDS-PAGE, nanoLC-MS, and glycoprotein analysis revealed at least eleven major protein bands, of which each morphological region contained different major proteins. Two glycoproteins, apyrase/5'-nucleotidase and D7, were identified. These results form a basis for further studies on details of cytopathological changes of malarial infected glands and roles of the proteins in disease transmission.
format Journal
author B. Phattanawiboon
N. Jariyapan
S. Roytrakul
A. Paemanee
S. Sor-suwan
N. Intakhan
W. Chanmol
P. Siriyasatien
A. Saeung
W. Choochote
author_facet B. Phattanawiboon
N. Jariyapan
S. Roytrakul
A. Paemanee
S. Sor-suwan
N. Intakhan
W. Chanmol
P. Siriyasatien
A. Saeung
W. Choochote
author_sort B. Phattanawiboon
title Morphological and protein analyses of adult female salivary glands of Anopheles barbirostris species A1 (Diptera: Culicidae)
title_short Morphological and protein analyses of adult female salivary glands of Anopheles barbirostris species A1 (Diptera: Culicidae)
title_full Morphological and protein analyses of adult female salivary glands of Anopheles barbirostris species A1 (Diptera: Culicidae)
title_fullStr Morphological and protein analyses of adult female salivary glands of Anopheles barbirostris species A1 (Diptera: Culicidae)
title_full_unstemmed Morphological and protein analyses of adult female salivary glands of Anopheles barbirostris species A1 (Diptera: Culicidae)
title_sort morphological and protein analyses of adult female salivary glands of anopheles barbirostris species a1 (diptera: culicidae)
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84936943361&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53708
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